RRC ID 47623
著者 Zhang Z, Liu Y, Wang S, Yang D, Cheng Y, Hu J, Chen J, Mei Y, Shen P, Bamford DH, Chen X.
タイトル Temperate membrane-containing halophilic archaeal virus SNJ1 has a circular dsDNA genome identical to that of plasmid pHH205.
ジャーナル Virology
Abstract A temperate haloarchaeal virus, SNJ1, was induced from the lysogenic host, Natrinema sp. J7-1, with mitomycin C, and the virus produced plaques on lawns of Natrinema sp. J7-2. Optimization of the induction conditions allowed us to increase the titer from ~10(4) PFU/ml to ~10(11) PFU/ml. Single-step growth curves exhibited a burst size of ~100 PFU/cell. The genome of SNJ1 was observed to be a circular, double-stranded DNA (dsDNA) molecule (16,341 bp). Surprisingly, the sequence of SNJ1 was identical to that of a previously described plasmid, pHH205, indicating that this plasmid is the provirus of SNJ1. Several structural protein-encoding genes were identified in the viral genome. In addition, the comparison of putative packaging ATPase sequences from bacterial, archaeal and eukaryotic viruses, as well as the presence of lipid constituents from the host phospholipid pool, strongly suggest that SNJ1 belongs to the PRD1-type lineage of dsDNA viruses, which have an internal membrane.
巻・号 434(2)
ページ 233-41
公開日 2012-12-20
DOI 10.1016/j.virol.2012.05.036
PII S0042-6822(12)00310-8
PMID 22784791
MeSH Amino Acid Sequence Archaeal Viruses / genetics* DNA / genetics* DNA, Circular / genetics DNA, Viral / genetics* Gene Order Genome, Viral* Halobacteriaceae / virology* Molecular Sequence Data Open Reading Frames Plasmids Proviruses / genetics* Sequence Analysis, DNA Viral Load Viral Plaque Assay Viral Proteins / genetics Virus Activation
IF 2.819
引用数 42
WOS 分野 VIROLOGY
リソース情報
一般微生物 JCM 8980 JCM 10476 JCM 14663