論文 - 詳細
| RRC ID | 47648 |
|---|---|
| 著者 | Satoh T, Nakamura Y, Satoh AK. |
| タイトル | The roles of Syx5 in Golgi morphology and Rhodopsin transport in Drosophila photoreceptors. |
| ジャーナル | Biol Open |
| Abstract |
SNAREs (SNAP receptors) are the key components of protein complexes that drive membrane fusion. Here, we report the function of a SNARE, Syntaxin 5 (Syx5), in the development of photoreceptors in Drosophila In wild-type photoreceptors, Syx5 localizes to cis-Golgi, along with cis-Golgi markers: Rab1 and GM130. We observed that Syx5-deficient photoreceptors show notable accumulation of these cis-Golgi markers accompanying drastic accumulation of vesicles between endoplasmic reticulum (ER) and Golgi cisternae. Extensive analysis of Rh1 (rhodopsin 1) trafficking revealed that in Syx5-deficient photoreceptors, Rh1 is exported from the ER with normal kinetics, retained in the cis-Golgi region along with GM130 for a prolonged period, and then subsequently degraded presumably by endoplasmic reticulum-associated protein degradation (ERAD) after retrieval to the ER. Unlike our previous report of Rab6-deficient photoreceptors - where two apical transport pathways are specifically inhibited - vesicle transport pathways to all plasma membrane domains are inhibited in Syx5-deficient photoreceptors, implying that Rab6 and Syx5 are acting in different steps of intra-Golgi transport. These results indicate that Syx5 is crucial for membrane protein transport, presumably during ER-derived vesicle fusion to form cis-Golgi cisternae. |
| 巻・号 | 5(10) |
| ページ | 1420-1430 |
| 公開日 | 2016-10-15 |
| DOI | 10.1242/bio.020958 |
| PII | bio.020958 |
| PMID | 27591190 |
| PMC | PMC5087674 |
| IF | 2.029 |
| 引用数 | 8 |
| WOS 分野 | BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#107139 |