論文 - 詳細
| RRC ID | 47649 |
|---|---|
| 著者 | Landeen EL, Muirhead CA, Wright L, Meiklejohn CD, Presgraves DC. |
| タイトル | Sex Chromosome-wide Transcriptional Suppression and Compensatory Cis-Regulatory Evolution Mediate Gene Expression in the Drosophila Male Germline. |
| ジャーナル | PLoS Biol |
| Abstract |
The evolution of heteromorphic sex chromosomes has repeatedly resulted in the evolution of sex chromosome-specific forms of regulation, including sex chromosome dosage compensation in the soma and meiotic sex chromosome inactivation in the germline. In the male germline of Drosophila melanogaster, a novel but poorly understood form of sex chromosome-specific transcriptional regulation occurs that is distinct from canonical sex chromosome dosage compensation or meiotic inactivation. Previous work shows that expression of reporter genes driven by testis-specific promoters is considerably lower-approximately 3-fold or more-for transgenes inserted into X chromosome versus autosome locations. Here we characterize this transcriptional suppression of X-linked genes in the male germline and its evolutionary consequences. Using transgenes and transpositions, we show that most endogenous X-linked genes, not just testis-specific ones, are transcriptionally suppressed several-fold specifically in the Drosophila male germline. In wild-type testes, this sex chromosome-wide transcriptional suppression is generally undetectable, being effectively compensated by the gene-by-gene evolutionary recruitment of strong promoters on the X chromosome. We identify and experimentally validate a promoter element sequence motif that is enriched upstream of the transcription start sites of hundreds of testis-expressed genes; evolutionarily conserved across species; associated with strong gene expression levels in testes; and overrepresented on the X chromosome. These findings show that the expression of X-linked genes in the Drosophila testes reflects a balance between chromosome-wide epigenetic transcriptional suppression and long-term compensatory adaptation by sex-linked genes. Our results have broad implications for the evolution of gene expression in the Drosophila male germline and for genome evolution. |
| 巻・号 | 14(7) |
| ページ | e1002499 |
| 公開日 | 2016-7-1 |
| DOI | 10.1371/journal.pbio.1002499 |
| PII | PBIOLOGY-D-16-00236 |
| PMID | 27404402 |
| PMC | PMC4942098 |
| MeSH | Animals Base Sequence Conserved Sequence Drosophila Proteins / biosynthesis Drosophila Proteins / genetics Drosophila melanogaster / genetics* Female Genes, Essential Genes, X-Linked Germ Cells / metabolism Male Promoter Regions, Genetic Testis / metabolism Transcription, Genetic* X Chromosome X Chromosome Inactivation* |
| IF | 7.076 |
| 引用数 | 18 |
| WOS 分野 | BIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 30 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#102067 |