論文 - 詳細
| RRC ID | 47683 |
|---|---|
| 著者 | Xiao Q, Zhang C, Li H, Wei B, Wang Y, Huang H, Li Y, Yu G, Liu H, Zhang J, Liu Y, Hu Y, Huang Y. |
| タイトル | Identification and functional analysis of the ICK gene family in maize. |
| ジャーナル | Sci Rep |
| Abstract |
Inhibitors of cyclin-dependent kinases (ICKs) are key regulators of cyclin-dependent kinase activities and cell division. Herein, we identified eight ICKs in maize, which we named Zeama;ICKs (ZmICKs). Primary sequencing and phylogenetic analyses were used to divide the ZmICK family into two classes: group B and group C. Subcellular localization analysis of ZmICK:enhanced green fluorescent protein (eGFP) fusion constructs in tobacco leaf cells indicated that ZmICKs are principally nuclear. Co-localization analysis of the ZmICKs and maize A-type cyclin-dependent kinase (ZmCDKA) was also performed using enhanced green fluorescent protein (eGFP) and red fluorescent protein (RFP) fusion constructs. The ZmICKs and ZmCDKA co-localized in the nucleus. Semi-quantitative RT-PCR analysis of the ZmICKs showed that they were expressed at different levels in all tissues examined and shared similar expression patterns with cell cycle-related genes. Yeast two-hybrid and bimolecular fluorescence complementation assays showed that ZmICK1, ZmICK2, ZmICK3, and ZmICK4 interact with ZmCDKA1 and ZmCDKA3. Interestingly, ZmICK7 interacts with D-type cyclins. Transformed and expressed ZmCDKA1 and ZmICKs together in fission yeast revealed that ZmICK1, ZmICK3, and ZmICK4 can affect ZmCDKA1 function. Moreover, the C-group of ZmICKs could interact with ZmCDKA1 directly and affect ZmCDKA1 function, suggesting that C-group ZmICKs are important for cell division regulation. |
| 巻・号 | 7 |
| ページ | 43818 |
| 公開日 | 2017-3-6 |
| DOI | 10.1038/srep43818 |
| PII | srep43818 |
| PMID | 28262730 |
| PMC | PMC5338338 |
| MeSH | Cell Cycle / genetics Cell Division / genetics Cell Nucleus / metabolism Cyclin-Dependent Kinase Inhibitor Proteins / chemistry Cyclin-Dependent Kinase Inhibitor Proteins / genetics* Cyclin-Dependent Kinase Inhibitor Proteins / metabolism Gene Expression Regulation, Plant* Luminescent Proteins / genetics Luminescent Proteins / metabolism Multigene Family* Phylogeny Plant Proteins / classification Plant Proteins / genetics* Plant Proteins / metabolism Plants, Genetically Modified Protein Binding Zea mays / genetics* Zea mays / metabolism |
| IF | 3.998 |
| 引用数 | 3 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 酵母 | FY12137 |