| RRC ID |
4770
|
| Author |
Kobayashi N, Noguchi H, Westerman KA, Watanabe T, Matsumura T, Totsugawa T, Fujiwara T, Leboulch P, Tanaka N.
|
| Title |
Cre/loxP-based reversible immortalization of human hepatocytes.
|
| Journal |
Cell Transplant
|
| Abstract |
An ideal alternative to the primary human hepatocytes for hepatocyte transplantation would be to use a clonal cell line that grows economically in culture and exhibits the characteristics of differentiated, nontransformed hepatocytes following transplantation. The purpose of the present studies was to establish a reversibly immortalized human hepatocyte cell line. Human hepatocytes were immortalized with a retroviral vector SSR#69 expressing simian virus 40 large T antigen (SV40Tag) gene flanked by a pair of loxP recombination targets. One of the resulting clones, NKNT-3, showed morphological characteristics of liver parenchymal cells and expressed the genes of differentiated liver functions. NKNT-3 cells offered unlimited availability. After an adenoviral delivery of Cre recombinase and subsequent differential selection, efficient removal of SV40Tag from NKNT-3 cells was performed. Here we represent that elimination of the retrovirally transferred SV40Tag gene can be excised by adenovirus-mediated site-specific recombination.
|
| Volume |
10(4-5)
|
| Pages |
383-6
|
| Published |
2001-1-1
|
| PMID |
11549058
|
| MeSH |
Cell Line*
Cell Transplantation
Culture Media, Serum-Free
Gene Transfer Techniques*
Genetic Vectors
Hepatocytes / physiology*
Hepatocytes / transplantation
Humans
Integrases / metabolism*
Liver / physiology
Recombination, Genetic
Retroviridae / genetics
Retroviridae / metabolism
Transduction, Genetic
Viral Proteins / metabolism*
|
| IF |
3.341
|
| Times Cited |
17
|
|
WOS Category
|
TRANSPLANTATION
MEDICINE, RESEARCH & EXPERIMENTAL
CELL & TISSUE ENGINEERING
|
| Resource |
| DNA material |
AxCALacZ (RDB1745) |
