論文 - 詳細
| RRC ID | 47727 |
|---|---|
| 著者 | Kusumi K, Yara A, Mitsui N, Tozawa Y, Iba K. |
| タイトル | Characterization of a rice nuclear-encoded plastid RNA polymerase gene OsRpoTp. |
| ジャーナル | Plant Cell Physiol |
| Abstract |
We isolated and characterized two rice genes, OsRpoTp and OsRpoTm, that encode putative phage-type RNA polymerases. Predicted amino acid sequences showed high homology of these genes to known RpoT genes. A transient expression assay using green fluorescent protein indicated that the encoded proteins were localized to plastids and mitochondria, respectively. We demonstrated by reverse transcription-PCR experiments and immunoblot analysis that OsRpoTp expression occurred at an early stage of leaf development, prior to the transcript accumulation of the genes that were transcribed by the nuclear-encoded plastid RNA polymerase (NEP). Expression analyses of the chloroplast-deficient rice mutant, virescent-1, showed a discrepancy between OsRpoTp protein accumulation and the level of transcripts of NEP-transcribed genes. Our results suggest that NEP activation is regulated by a process after transcription, and is affected by the developmental state of chloroplast biogenesis. |
| 巻・号 | 45(9) |
| ページ | 1194-201 |
| 公開日 | 2004-9-1 |
| DOI | 10.1093/pcp/pch133 |
| PII | 45/9/1194 |
| PMID | 15509842 |
| MeSH | Base Sequence Cell Nucleus / enzymology* Cloning, Molecular DNA Primers DNA-Directed RNA Polymerases / genetics* Molecular Sequence Data Oryza / enzymology* Oryza / genetics Oryza / growth & development Plant Leaves / enzymology Plant Leaves / growth & development Plastids / genetics* Recombinant Proteins / genetics Reverse Transcriptase Polymerase Chain Reaction |
| IF | 4.062 |
| 引用数 | 47 |
| WOS 分野 | PLANT SCIENCES CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| イネ | 標識遺伝子系統 |