Reference - Detail
RRC ID | 47769 |
---|---|
Author | Shimatani Z, Kashojiya S, Takayama M, Terada R, Arazoe T, Ishii H, Teramura H, Yamamoto T, Komatsu H, Miura K, Ezura H, Nishida K, Ariizumi T, Kondo A. |
Title | Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. |
Journal | Nat Biotechnol |
Abstract |
We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement. |
Volume | 35(5) |
Pages | 441-443 |
Published | 2017-5-1 |
DOI | 10.1038/nbt.3833 |
PII | nbt.3833 |
PMID | 28346401 |
MeSH | Base Pairing / genetics CRISPR-Associated Proteins / genetics* Clustered Regularly Interspaced Short Palindromic Repeats / genetics Cytidine Deaminase / genetics DNA, Plant / genetics* Gene Editing / methods* Genes, Plant / genetics Mutagenesis, Site-Directed / methods* Oryza / genetics* Plants, Genetically Modified / genetics Point Mutation / genetics Recombinant Fusion Proteins / genetics Solanum lycopersicum / genetics* |
IF | 36.553 |
Times Cited | 189 |
WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
Resource | |
Tomato | TOMJPF00001 |