Reference - Detail
| RRC ID | 477 |
|---|---|
| Author | Daimon T, Hamada K, Mita K, Okano K, Suzuki MG, Kobayashi M, Shimada T. |
| Title | A Bombyx mori gene, BmChi-h, encodes a protein homologous to bacterial and baculovirus chitinases. |
| Journal | Insect Biochem Mol Biol |
| Abstract |
We have cloned and characterized a novel chitinase gene (BmChi-h) from the silkworm, Bombyx mori. BmChi-h cDNA has an open reading frame of 1,665 nucleotides, encoding a protein of 555 amino acid residues. The predicted protein shared extensive similarities with bacterial and baculovirus chitinases in both amino acid sequences (73% identity with Serratia marcescens chiA and 63% with Autographa californica nucleopolyhedrovirus chiA) and domain architectures. BmChi-h was a single-copy gene and located on chromosome 7. The expression of BmChi-h mRNA was observed in a stage- and tissue-specific manner that was almost identical to that of another chitinase gene previously cloned from B. mori. We further determined the overall genomic organization of BmChi-h. There was no intron in the ORF of BmChi-h. However, BmChi-h was transcribed from three promoters, which generated three isoforms in the 5'-UTR of the transcript. Phylogenetic analysis suggested that ancestral species of B. mori acquired the chitinase gene from a bacterium or an ancestral baculovirus via horizontal gene transfer. |
| Volume | 33(8) |
| Pages | 749-59 |
| Published | 2003-8-1 |
| DOI | 10.1016/s0965-1748(03)00084-5 |
| PII | S0965174803000845 |
| PMID | 12878222 |
| MeSH | Amino Acid Sequence Animals Bacterial Proteins / genetics* Baculoviridae / chemistry Baculoviridae / genetics* Base Sequence Biological Evolution Blotting, Northern Blotting, Southern Bombyx / genetics* Chitinases / genetics* Cloning, Molecular DNA, Complementary Gene Transfer, Horizontal Insect Proteins / genetics* Molecular Sequence Data Phylogeny Polymerase Chain Reaction Sequence Homology, Amino Acid |
| IF | 3.827 |
| Times Cited | 62 |
| WOS Category | ENTOMOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Silkworms | p50 |