| RRC ID |
477
|
| Author |
Daimon T, Hamada K, Mita K, Okano K, Suzuki MG, Kobayashi M, Shimada T.
|
| Title |
A Bombyx mori gene, BmChi-h, encodes a protein homologous to bacterial and baculovirus chitinases.
|
| Journal |
Insect Biochem Mol Biol
|
| Abstract |
We have cloned and characterized a novel chitinase gene (BmChi-h) from the silkworm, Bombyx mori. BmChi-h cDNA has an open reading frame of 1,665 nucleotides, encoding a protein of 555 amino acid residues. The predicted protein shared extensive similarities with bacterial and baculovirus chitinases in both amino acid sequences (73% identity with Serratia marcescens chiA and 63% with Autographa californica nucleopolyhedrovirus chiA) and domain architectures. BmChi-h was a single-copy gene and located on chromosome 7. The expression of BmChi-h mRNA was observed in a stage- and tissue-specific manner that was almost identical to that of another chitinase gene previously cloned from B. mori. We further determined the overall genomic organization of BmChi-h. There was no intron in the ORF of BmChi-h. However, BmChi-h was transcribed from three promoters, which generated three isoforms in the 5'-UTR of the transcript. Phylogenetic analysis suggested that ancestral species of B. mori acquired the chitinase gene from a bacterium or an ancestral baculovirus via horizontal gene transfer.
|
| Volume |
33(8)
|
| Pages |
749-59
|
| Published |
2003-8-1
|
| DOI |
10.1016/s0965-1748(03)00084-5
|
| PII |
S0965174803000845
|
| PMID |
12878222
|
| MeSH |
Amino Acid Sequence
Animals
Bacterial Proteins / genetics*
Baculoviridae / chemistry
Baculoviridae / genetics*
Base Sequence
Biological Evolution
Blotting, Northern
Blotting, Southern
Bombyx / genetics*
Chitinases / genetics*
Cloning, Molecular
DNA, Complementary
Gene Transfer, Horizontal
Insect Proteins / genetics*
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Sequence Homology, Amino Acid
|
| IF |
3.827
|
| Times Cited |
62
|
|
WOS Category
|
ENTOMOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Silkworms |
p50 |
