Reference - Detail
| RRC ID | 47900 |
|---|---|
| Author | Anas AR, Nakajima A, Naruse C, Tone M, Asukabe H, Harada K. |
| Title | Determination of FVIIa-sTF Inhibitors in Toxic Microcystis Cyanobacteria by LC-MS Technique. |
| Journal | Mar Drugs |
| Abstract |
The blood coagulation cascade involves the human coagulation factors thrombin and an activated factor VII (fVIIa). Thrombin and fVIIa are vitamin-K-dependent clotting factors associated with bleeding, bleeding complications and disorders. Thrombin and fVIIa cause excessive bleeding when treated with vitamin-K antagonists. In this research, we explored different strains of toxic Microcystis aeruginosa and cyanobacteria blooms for the probable fVIIa-soluble Tissue Factor (fVIIa-sTF) inhibitors. The algal cells were subjected to acidification, and reverse phase (ODS) chromatography-solid phase extraction eluted by water to 100% MeOH with 20%-MeOH increments except for M. aeruginosa NIES-89, from the National Institute for Environmental Studies (NIES), which was eluted with 5%-MeOH increments as an isolation procedure to separate aeruginosins 89A and B from co-eluting microcystins. The 40%-80% MeOH fractions of the cyanobacterial extract are active against fVIIa-sTF. The fVIIa-sTF active fractions from cultured cyanobacteria and cyanobacteria blooms were subjected to liquid chromatography-mass spectrometry (LC-MS). The 60% MeOH fraction of M. aeruginosa K139 exhibited an m/z 603 [M + H]⁺ attributed to aeruginosin K139, and the 40% MeOH fraction of M. aeruginosa NIES-89 displayed ions with m/z 617 [M - SO3 + H]⁺ and m/z [M + H]⁺ 717, which attributed to aeruginosin 89. Aeruginosins 102A/B and 298A/B were also observed from other toxic strains of M. aeruginosa with positive fVIIa-sTF inhibitory activity. The active fractions contained cyanobacterial peptides of the aeruginosin class as fVIIa-sTF inhibitors detected by LC-MS. |
| Volume | 14(1) |
| Pages | 7 |
| Published | 2015-12-30 |
| DOI | 10.3390/md14010007 |
| PII | md14010007 |
| PMID | 26729138 |
| PMC | PMC4728504 |
| MeSH | Anticoagulants / pharmacology* Chromatography, Liquid Cyanobacteria / chemistry* Factor VIIa / antagonists & inhibitors* Factor VIIa / drug effects Fresh Water Humans Japan Mass Spectrometry Structure-Activity Relationship Thromboplastin / antagonists & inhibitors* Thromboplastin / drug effects |
| IF | 4.073 |
| Times Cited | 4 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Algae | NIES-89 NIES-102 NIES-103 NIES-107 NIES-1025 NIES-1058 NIES-1071 NIES-1085 NIES-1099 NIES-1133 NIES-1043 NIES-298 |