RRC ID 47920
Author Sun Z, Amourda C, Shagirov M, Hara Y, Saunders TE, Toyama Y.
Title Basolateral protrusion and apical contraction cooperatively drive Drosophila germ-band extension.
Journal Nat Cell Biol
Abstract Throughout development, tissues undergo complex morphological changes, resulting from cellular mechanics that evolve over time and in three-dimensional space. During Drosophila germ-band extension (GBE), cell intercalation is the key mechanism for tissue extension, and the associated apical junction remodelling is driven by polarized myosin-II-dependent contraction. However, the contribution of the basolateral cellular mechanics to GBE remains poorly understood. Here, we characterize how cells coordinate their shape from the apical to the basal side during rosette formation, a hallmark of cell intercalation. Basolateral rosette formation is driven by cells mostly located at the dorsal/ventral part of the rosette (D/V cells). These cells exhibit actin-rich wedge-shaped basolateral protrusions and migrate towards each other. Surprisingly, the formation of basolateral rosettes precedes that of the apical rosettes. Basolateral rosette formation is independent of apical contractility, but requires Rac1-dependent protrusive motility. Furthermore, we identified Src42A as a regulator of basolateral rosette formation. Our data show that in addition to apical contraction, active cell migration driven by basolateral protrusions plays a pivotal role in rosette formation and contributes to GBE.
Volume 19(4)
Pages 375-383
Published 2017-4-1
DOI 10.1038/ncb3497
PII ncb3497
PMID 28346438
MeSH Actins / metabolism Animals Body Patterning* Cell Polarity* Drosophila Proteins / metabolism Drosophila melanogaster / cytology* Drosophila melanogaster / embryology* Embryo, Nonmammalian / cytology Microscopy, Fluorescence, Multiphoton Phosphatidylinositol Phosphates / metabolism Time-Lapse Imaging rac GTP-Binding Proteins / metabolism
IF 20.042
Times Cited 35
WOS Category CELL BIOLOGY
Resource
Drosophila 7873R-2