論文 - 詳細
| RRC ID | 47920 |
|---|---|
| 著者 | Sun Z, Amourda C, Shagirov M, Hara Y, Saunders TE, Toyama Y. |
| タイトル | Basolateral protrusion and apical contraction cooperatively drive Drosophila germ-band extension. |
| ジャーナル | Nat Cell Biol |
| Abstract |
Throughout development, tissues undergo complex morphological changes, resulting from cellular mechanics that evolve over time and in three-dimensional space. During Drosophila germ-band extension (GBE), cell intercalation is the key mechanism for tissue extension, and the associated apical junction remodelling is driven by polarized myosin-II-dependent contraction. However, the contribution of the basolateral cellular mechanics to GBE remains poorly understood. Here, we characterize how cells coordinate their shape from the apical to the basal side during rosette formation, a hallmark of cell intercalation. Basolateral rosette formation is driven by cells mostly located at the dorsal/ventral part of the rosette (D/V cells). These cells exhibit actin-rich wedge-shaped basolateral protrusions and migrate towards each other. Surprisingly, the formation of basolateral rosettes precedes that of the apical rosettes. Basolateral rosette formation is independent of apical contractility, but requires Rac1-dependent protrusive motility. Furthermore, we identified Src42A as a regulator of basolateral rosette formation. Our data show that in addition to apical contraction, active cell migration driven by basolateral protrusions plays a pivotal role in rosette formation and contributes to GBE. |
| 巻・号 | 19(4) |
| ページ | 375-383 |
| 公開日 | 2017-4-1 |
| DOI | 10.1038/ncb3497 |
| PII | ncb3497 |
| PMID | 28346438 |
| MeSH | Actins / metabolism Animals Body Patterning* Cell Polarity* Drosophila Proteins / metabolism Drosophila melanogaster / cytology* Drosophila melanogaster / embryology* Embryo, Nonmammalian / cytology Microscopy, Fluorescence, Multiphoton Phosphatidylinositol Phosphates / metabolism Time-Lapse Imaging rac GTP-Binding Proteins / metabolism |
| IF | 20.042 |
| 引用数 | 35 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 347 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 7873R-2 |