RRC ID 47946
著者 Aliyeva-Schnorr L, Stein N, Houben A.
タイトル Collinearity of homoeologous group 3 chromosomes in the genus Hordeum and Secale cereale as revealed by 3H-derived FISH analysis.
ジャーナル Chromosome Res.
Abstract Crop wild relatives are considered as important genetic resources of allelic diversity for domesticated crop species. Their utilization in breeding programs, however, is often limited due to crossing barriers and genome incompatibilities. Wild relatives of barley possess attractive properties and hence allelic diversity for adapting barley better to changing environmental conditions. Therefore, gaining a better knowledge about genomic synteny between cultivated barley and wild relatives of the same genus is an important task. To visualize genomic collinearity in related species, 22 genomic single-copy and 14 complementary DNA (cDNA) chromosome 3H-specific probes were mapped to the chromosomes of Hordeum bulbosum, Hordeum marinum, Hordeum pubiflorum, Hordeum murinum, and Secale cereale by fluorescent in situ hybridization (FISH). Most probes showed reliable signals confirming homoeology between cultivated barley and related species. Differences in order and position of FISH markers demonstrated sequence movements or small-scale chromosomal rearrangements within genus Hordeum and confirmed interchromosomal rearrangements between barley and rye. Comparison between repeat-free genomic and cDNA probes showed that gene-containing single-copy genomic DNA (gDNA) probes are performing more reliably for FISH-based analysis of synteny.
巻・号 24(2)
ページ 231-42
公開日 2016-5
DOI 10.1007/s10577-016-9518-8
PII 10.1007/s10577-016-9518-8
PMID 26883649
MeSH Base Sequence Chromosome Mapping / methods* Chromosomes, Plant / genetics* DNA Probes / genetics DNA, Plant / genetics* Expressed Sequence Tags Hordeum / genetics* In Situ Hybridization, Fluorescence Secale / genetics* Synteny / genetics*
IF 2.385
引用数 2
WOS 分野 GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
オオムギ FLcDNA: AK251893 AK248957 AK251014 AK251696 AK249216 AK336104 AK248727 AK248230 AK251227