RRC ID 47947
Author Hisano H, Meints B, Moscou MJ, Cistue L, Echávarri B, Sato K, Hayes PM.
Title Selection of transformation-efficient barley genotypes based on TFA (transformation amenability) haplotype and higher resolution mapping of the TFA loci.
Journal Plant Cell Rep.
Abstract KEY MESSAGE:The genetic substitution of transformation amenability alleles from 'Golden Promise' can facilitate the development of transformation-efficient lines from recalcitrant barley cultivars. Barley (Hordeum vulgare) cv. 'Golden Promise' is one of the most useful and well-studied cultivars for genetic manipulation. In a previous report, we identified several transformation amenability (TFA) loci responsible for Agrobacterium-mediated transformation using the F2 generation of immature embryos, derived from 'Haruna Nijo' × 'Golden Promise,' as explants. In this report, we describe higher density mapping of these TFA regions with additional SNP markers using the same transgenic plants. To demonstrate the robustness of transformability alleles at the TFA loci, we genotyped 202 doubled haploid progeny from the cross 'Golden Promise' × 'Full Pint.' Based on SNP genotype, we selected lines having 'Golden Promise' alleles at TFA loci and used them for transformation. Of the successfully transformed lines, DH120366 came the closest to achieving a level of transformation efficiency comparable to 'Golden Promise.' The results validate that the genetic substitution of TFA alleles from 'Golden Promise' can facilitate the development of transformation-efficient lines from recalcitrant barley cultivars.
Volume 36(4)
Pages 611-620
Published 2017-4
DOI 10.1007/s00299-017-2107-2
PII 10.1007/s00299-017-2107-2
PMID 28204911
MeSH Agrobacterium tumefaciens / genetics Chromosome Mapping Genotype Haploidy Haplotypes / genetics* Hordeum / genetics* Plant Proteins / genetics* Seeds / genetics Transformation, Genetic / genetics
IF 2.989
Times Cited 0
WOS Category PLANT SCIENCES
Resource
Barley Haruna Nijo Golden Promise