論文 - 詳細
| RRC ID | 47947 |
|---|---|
| 著者 | Hisano H, Meints B, Moscou MJ, Cistue L, Echávarri B, Sato K, Hayes PM. |
| タイトル | Selection of transformation-efficient barley genotypes based on TFA (transformation amenability) haplotype and higher resolution mapping of the TFA loci. |
| ジャーナル | Plant Cell Rep |
| Abstract |
KEY MESSAGE:The genetic substitution of transformation amenability alleles from 'Golden Promise' can facilitate the development of transformation-efficient lines from recalcitrant barley cultivars. Barley (Hordeum vulgare) cv. 'Golden Promise' is one of the most useful and well-studied cultivars for genetic manipulation. In a previous report, we identified several transformation amenability (TFA) loci responsible for Agrobacterium-mediated transformation using the F2 generation of immature embryos, derived from 'Haruna Nijo' × 'Golden Promise,' as explants. In this report, we describe higher density mapping of these TFA regions with additional SNP markers using the same transgenic plants. To demonstrate the robustness of transformability alleles at the TFA loci, we genotyped 202 doubled haploid progeny from the cross 'Golden Promise' × 'Full Pint.' Based on SNP genotype, we selected lines having 'Golden Promise' alleles at TFA loci and used them for transformation. Of the successfully transformed lines, DH120366 came the closest to achieving a level of transformation efficiency comparable to 'Golden Promise.' The results validate that the genetic substitution of TFA alleles from 'Golden Promise' can facilitate the development of transformation-efficient lines from recalcitrant barley cultivars. |
| 巻・号 | 36(4) |
| ページ | 611-620 |
| 公開日 | 2017-4-1 |
| DOI | 10.1007/s00299-017-2107-2 |
| PII | 10.1007/s00299-017-2107-2 |
| PMID | 28204911 |
| MeSH | Agrobacterium tumefaciens / genetics Chromosome Mapping Genotype Haploidy Haplotypes / genetics* Hordeum / genetics* Plant Proteins / genetics* Seeds / genetics Transformation, Genetic / genetics |
| IF | 3.825 |
| 引用数 | 8 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| オオムギ | Haruna Nijo Golden Promise |