RRC ID 47962
Author Wang D, Li Q, Yang Y, Hao S, Han X, Song J, Yin Y, Li X, Tanaka M, Qiu CH.
Title Macrophage Subset Expressing CD169 in Peritoneal Cavity-Regulated Mucosal Inflammation Together with Lower Levels of CCL22.
Journal Inflammation
Abstract Crohn's disease (CD) and ulcerative colitis (UC) are the most widely known types of inflammatory bowel diseases (IBD) and have been paid more attention due to their increasing incidence and a substantial increase in the risk of colorectal cancer (CRC). However, the phenotype and, more importantly, the function in the regulation of mucosal inflammation by different macrophages are poorly understood, even though macrophages constitute a major subset of intestinal myeloid cells. The results firstly showed that the subset of peritoneal CD11b+CD169+ macrophages increased and CCL22 expression level decreased significantly during the DSS-induced colitis. DSS-induced colitis was alleviated in CD169-DTR mice at least partially due to the deletion CD169+ macrophages. Moreover, the CCL22 expression level in peritoneal macrophages from CD169-DTR mice was much higher than that from WT mice with DSS-induced colitis. And, the cell-sorting result revealed that CD11b+CD169+ macrophage cells did not express CCL22 dominantly. Further experiment in vivo demonstrated that treatment with recombinant murine CCL22 (rmCCL22) ameliorated the clinical symptoms of DSS-induced colitis. All these data indicated that macrophage subset of CD11b+CD169+ from peritoneal cavity played critical role probably together with low levels of CCL22 in DSS-induced colitis.
Volume 40(4)
Pages 1191-1203
Published 2017-8-1
DOI 10.1007/s10753-017-0562-0
PII 10.1007/s10753-017-0562-0
PMID 28466432
MeSH Animals Chemokine CCL22 / analysis Chemokine CCL22 / metabolism* Colitis / chemically induced* Dextran Sulfate Inflammation* Macrophages, Peritoneal / metabolism* Mice Mucositis Peritoneal Cavity / pathology* Sialic Acid Binding Ig-like Lectin 1 / analysis* Sialic Acid Binding Ig-like Lectin 1 / metabolism
IF 3.212
Times Cited 2
Mice RBRC04395