Reference - Detail
| RRC ID | 48010 |
|---|---|
| Author | Katafuchi Y, Li Q, Tanaka Y, Shinozuka S, Kawamitsu Y, Izumi M, Ekino K, Mizuki K, Takegawa K, Shibata N, Goto M, Nomura Y, Ohta K, Oka T. |
| Title | GfsA is a β1,5-galactofuranosyltransferase involved in the biosynthesis of the galactofuran side chain of fungal-type galactomannan in Aspergillus fumigatus. |
| Journal | Glycobiology |
| Abstract |
Previously, we reported that GfsA is a novel galactofuranosyltransferase involved in the biosynthesis of O-glycan, the proper maintenance of fungal morphology, the formation of conidia and anti-fungal resistance in Aspergillus nidulans and A. fumigatus (Komachi Y et al., 2013. GfsA encodes a novel galactofuranosyltransferase involved in biosynthesis of galactofuranose antigen of O-glycan in Aspergillus nidulans and Aspergillus fumigatus. Mol. Microbiol. 90:1054-1073). In the present paper, to gain an in depth-understanding of the enzymatic functions of GfsA in A. fumigatus (AfGfsA), we established an in vitro assay to measure galactofuranosyltransferase activity using purified AfGfsA, UDP-α-d-galactofuranose as a sugar donor, and p-nitrophenyl-β-d-galactofuranoside as an acceptor substrate. LC/MS, 1H-NMR and methylation analyses of the enzymatic products of AfGfsA revealed that this protein has the ability to transfer galactofuranose to the C-5 position of the β-galactofuranose residue via a β-linkage. AfGfsA requires a divalent cation of manganese for maximal activity and consumes UDP-α-d-galactofuranose as a sugar donor. Its optimal pH range is 6.5-7.5 and its optimal temperature range is 20-30°C. 1H-NMR, 13C-NMR and methylation analyses of fungal-type galactomannan extracted from the ∆AfgfsA strain revealed that AfGfsA is responsible for the biosynthesis of β1,5-galactofuranose in the galactofuran side chain of fungal-type galactomannan. Based on these results, we conclude that AfGfsA acts as a UDP-α-d-galactofuranose: β-d-galactofuranoside β1,5-galactofuranosyltransferase in the biosynthetic pathway of galactomannans. |
| Volume | 27(6) |
| Pages | 568-581 |
| Published | 2017-6-1 |
| DOI | 10.1093/glycob/cwx028 |
| PII | 3091656 |
| PMID | 28369326 |
| MeSH | Aspergillus fumigatus / enzymology* Fungal Polysaccharides / chemistry Fungal Polysaccharides / metabolism* Fungal Proteins / chemistry Fungal Proteins / genetics Fungal Proteins / metabolism* Furans / chemistry Furans / metabolism Galactose / analogs & derivatives Galactosyltransferases / chemistry Galactosyltransferases / genetics Galactosyltransferases / metabolism* Manganese / chemistry Mannans / chemistry Mannans / metabolism |
| IF | 4.06 |
| Times Cited | 14 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | JW2021-AM |