RRC ID 48066
Author Tsuji S, Washimi K, Kageyama T, Yamashita M, Yoshihara M, Matsuura R, Yokose T, Kameda Y, Hayashi H, Morohoshi T, Tsuura Y, Yusa T, Sato T, Togayachi A, Narimatsu H, Nagasaki T, Nakamoto K, Moriwaki Y, Misawa H, Hiroshima K, Miyagi Y, Imai K.
Title HEG1 is a novel mucin-like membrane protein that serves as a diagnostic and therapeutic target for malignant mesothelioma.
Journal Sci Rep
Abstract The absence of highly specific markers for malignant mesothelioma (MM) has served an obstacle for its diagnosis and development of molecular-targeting therapy against MM. Here, we show that a novel mucin-like membrane protein, sialylated protein HEG homolog 1 (HEG1), is a highly specific marker for MM. A monoclonal antibody against sialylated HEG1, SKM9-2, can detect even sarcomatoid and desmoplastic MM. The specificity and sensitivity of SKM9-2 to MM reached 99% and 92%, respectively; this antibody did not react with normal tissues. This accurate discrimination by SKM9-2 was due to the recognition of a sialylated O-linked glycan with HEG1 peptide. We also found that gene silencing of HEG1 significantly suppressed the survival and proliferation of mesothelioma cells; this result suggests that HEG1 may be a worthwhile target for function-inhibition drugs. Taken together, our results indicate that sialylated HEG1 may be useful as a diagnostic and therapeutic target for MM.
Volume 7
Pages 45768
Published 2017-3-31
DOI 10.1038/srep45768
PII srep45768
PMID 28361969
PMC PMC5374711
MeSH Antibodies, Monoclonal / immunology* Antibodies, Monoclonal / metabolism Biomarkers, Tumor / immunology Biomarkers, Tumor / metabolism Cell Line, Tumor Cell Proliferation Glycosylation Humans Lung Neoplasms / diagnosis* Lung Neoplasms / immunology* Lung Neoplasms / metabolism Membrane Proteins / genetics Membrane Proteins / immunology* Membrane Proteins / metabolism Mesothelioma / diagnosis* Mesothelioma / immunology* Mesothelioma / metabolism Mesothelioma, Malignant Sensitivity and Specificity
IF 3.998
Times Cited 16
Human and Animal Cells ACC-MESO-1(RCB2292) ACC-MESO-4(RCB2293)