RRC ID 48067
Author Endo S, Nakata K, Ohuchida K, Takesue S, Nakayama H, Abe T, Koikawa K, Okumura T, Sada M, Horioka K, Zheng B, Mizuuchi Y, Iwamoto C, Murata M, Moriyama T, Miyasaka Y, Ohtsuka T, Mizumoto K, Oda Y, Hashizume M, Nakamura M.
Title Autophagy Is Required for Activation of Pancreatic Stellate Cells, Associated With Pancreatic Cancer Progression and Promotes Growth of Pancreatic Tumors in Mice.
Journal Gastroenterology
Abstract BACKGROUND & AIMS:Pancreatic stellate cells (PSCs) change from a quiescent to activated state in the tumor environment and secrete extracellular matrix (ECM) molecules and cytokines to increase the aggressiveness of tumors. However, it is not clear how PSCs are activated to produce these factors, or whether this process can be inhibited. PSCs have morphologic and functional similarities to hepatic stellate cells, which undergo autophagy to promote fibrosis and tumor growth. We investigated whether autophagy activates PSCs, which promotes development of the tumor stroma and growth of pancreatic tumors in mice.
METHODS:We used immunofluorescence microscopy and immunohistochemistry to analyze pancreatic tumor specimens from 133 patients who underwent pancreatectomy in Japan from 2000 to 2009. PSCs were cultured from pancreatic tumor tissues or tissues of patients with chronic pancreatitis; these were analyzed by immunofluorescence microscopy, immunoblots, quantitative reverse transcription polymerase chain reaction, and in assays for invasiveness, proliferation, and lipid droplets. Autophagy was inhibited in PSCs by administration of chloroquine or transfection with small interfering RNAs. Proteins were knocked down in immortalized PSCs by expression of small hairpin RNAs. Cells were transplanted into pancreatic tails of nude mice, and tumor growth and metastasis were quantified.
RESULTS:Based on immunohistochemical analyses, autophagy was significantly associated with tumor T category (P = .018), histologic grade (P = .001), lymph node metastases (P < .001), stage (P = .009), perilymphatic invasion (P = .001), and perivascular invasion (P = .003). Autophagy of PSCs was associated with shorter survival times of patients with pancreatic cancer. PSC expression of microtubule-associated protein 1 light chain 3, a marker of autophagosomes, was associated with poor outcomes (shorter survival time, disease recurrence) for patients with pancreatic cancer (relative risk of shorter survival time, 1.56). Immunoblots showed that PSCs from pancreatic tumor samples expressed higher levels of markers of autophagy than PSCs from chronic pancreatitis samples. Inhibitors of autophagy increased the number of lipid droplets of PSCs, indicating a quiescent state of PSCs, and reduced their production of ECM molecules and interleukin 6, as well as their proliferation and invasiveness in culture. PSCs exposed to autophagy inhibitors formed smaller tumors in nude mice (P = .001) and fewer liver metastases (P = .018) with less peritoneal dissemination (P = .018) compared to PSCs not exposed to autophagy inhibitors.
CONCLUSIONS:Autophagic PSCs produce ECM molecules and interleukin 6 and are associated with shorter survival times and disease recurrence in patients with pancreatic cancer. Inhibitors of PSC autophagy might reduce pancreatic tumor invasiveness by altering the tumor stroma.
Volume 152(6)
Pages 1492-1506.e24
Published 2017-5-1
DOI 10.1053/j.gastro.2017.01.010
PII S0016-5085(17)30050-1
PMID 28126348
MeSH Animals Autophagy* / drug effects Autophagy* / genetics Cell Line, Tumor Cell Movement Cell Proliferation Chloroquine / pharmacology Disease Progression Extracellular Matrix / metabolism* Female Humans Immunohistochemistry Interleukin-6 / metabolism* Lipid Droplets Lymphatic Metastasis Male Mice Mice, Inbred BALB C Microscopy, Fluorescence Microtubule-Associated Proteins / metabolism Neoplasm Grading Neoplasm Invasiveness Neoplasm Staging Neoplasm Transplantation Pancreatic Neoplasms / chemistry Pancreatic Neoplasms / diagnostic imaging Pancreatic Neoplasms / pathology* Pancreatic Neoplasms / physiopathology* Pancreatic Stellate Cells / metabolism Pancreatic Stellate Cells / physiology* Pancreatitis, Chronic / diagnostic imaging Pancreatitis, Chronic / metabolism RNA, Small Interfering / genetics Survival Rate Transfection
IF 19.809
Times Cited 59
Human and Animal Cells PANC-1(RCB2095) MRC-5(RCB0211)