RRC ID 48079
Author Shen L, Nishimura Y, Matsuda F, Ishii J, Kondo A.
Title Overexpressing enzymes of the Ehrlich pathway and deleting genes of the competing pathway in Saccharomyces cerevisiae for increasing 2-phenylethanol production from glucose.
Journal J Biosci Bioeng
Abstract 2-Phenylethanol (2-PE) is a higher aromatic alcohol that is used in the cosmetics and food industries. The budding yeast Saccharomyces cerevisiae is considered to be a suitable host for the industrial production of higher alcohols, including 2-PE. To produce 2-PE from glucose in S. cerevisiae, we searched for suitable 2-keto acid decarboxylase (KDC) and alcohol dehydrogenase (ADH) enzymes of the Ehrlich pathway for overexpression in strain YPH499, and found that overexpression of the ARO10 and/or ADH1 genes increased 2-PE production from glucose. Further, we screened ten BY4741 single-deletion mutants of genes involved in the competing pathways for 2-PE production, and found that strains aro8Δ and aat2Δ displayed increased 2-PE production. Based on these results, we engineered a BY4741 strain that overexpressed ARO10 and contained an aro8Δ deletion, and demonstrated that the strain produced 96 mg/L 2-PE from glucose as the sole carbon source. As this engineered S. cerevisiae strain showed a significant increase in 2-PE production from glucose without the addition of an intermediate carbon substrate, it is a promising candidate for the large-scale production of 2-PE.
Volume 122(1)
Pages 34-9
Published 2016-7-1
DOI 10.1016/j.jbiosc.2015.12.022
PII S1389-1723(16)00044-X
PMID 26975754
MeSH Alcohol Dehydrogenase / genetics Alcohol Dehydrogenase / metabolism Bioreactors* Biosynthetic Pathways / genetics Gene Deletion* Glucose / metabolism* Metabolic Engineering* Phenylethyl Alcohol / metabolism* Saccharomyces cerevisiae / enzymology Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism*
IF 2.366
Times Cited 13
Yeast pGK424 pGK425 pGK426