論文 - 詳細
| RRC ID | 48079 |
|---|---|
| 著者 | Shen L, Nishimura Y, Matsuda F, Ishii J, Kondo A. |
| タイトル | Overexpressing enzymes of the Ehrlich pathway and deleting genes of the competing pathway in Saccharomyces cerevisiae for increasing 2-phenylethanol production from glucose. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
2-Phenylethanol (2-PE) is a higher aromatic alcohol that is used in the cosmetics and food industries. The budding yeast Saccharomyces cerevisiae is considered to be a suitable host for the industrial production of higher alcohols, including 2-PE. To produce 2-PE from glucose in S. cerevisiae, we searched for suitable 2-keto acid decarboxylase (KDC) and alcohol dehydrogenase (ADH) enzymes of the Ehrlich pathway for overexpression in strain YPH499, and found that overexpression of the ARO10 and/or ADH1 genes increased 2-PE production from glucose. Further, we screened ten BY4741 single-deletion mutants of genes involved in the competing pathways for 2-PE production, and found that strains aro8Δ and aat2Δ displayed increased 2-PE production. Based on these results, we engineered a BY4741 strain that overexpressed ARO10 and contained an aro8Δ deletion, and demonstrated that the strain produced 96 mg/L 2-PE from glucose as the sole carbon source. As this engineered S. cerevisiae strain showed a significant increase in 2-PE production from glucose without the addition of an intermediate carbon substrate, it is a promising candidate for the large-scale production of 2-PE. |
| 巻・号 | 122(1) |
| ページ | 34-9 |
| 公開日 | 2016-7-1 |
| DOI | 10.1016/j.jbiosc.2015.12.022 |
| PII | S1389-1723(16)00044-X |
| PMID | 26975754 |
| MeSH | Alcohol Dehydrogenase / genetics Alcohol Dehydrogenase / metabolism Bioreactors* Biosynthetic Pathways / genetics Gene Deletion* Glucose / metabolism* Metabolic Engineering* Phenylethyl Alcohol / metabolism* Saccharomyces cerevisiae / enzymology Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* |
| IF | 2.366 |
| 引用数 | 13 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY FOOD SCIENCE & TECHNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | pGK424 pGK425 pGK426 |