RRC ID 48107
Author Karp SJ, Masu M, Eki T, Ozawa K, Nakanishi S.
Title Molecular cloning and chromosomal localization of the key subunit of the human N-methyl-D-aspartate receptor.
Journal J Biol Chem
Abstract A complementary DNA encoding the key subunit of the human N-methyl-D-aspartate (NMDA) receptor (NMDAR1) has been cloned using a probe derived from the rat NMDAR1 cDNA. The cDNA encodes a 938-amino acid protein, which shows 99% amino acid homology with the rat counterpart. Of the 7 of 938 amino acids which are different, three occur in the region of the signal peptide and the others in the extracellular amino-terminal domain preceding the 4 putative transmembrane segments. Expression in Xenopus oocytes demonstrated that the single protein encoded by the cloned cDNA possesses the electrophysiological and pharmacological properties characteristic of the NMDA receptor, including Ca2+ permeability, voltage-dependent Mg2+ block, and inhibition by selective antagonists such as Zn2+ and channel blockers. The high evolutionary conservation in the structure and properties of NMDAR1 argues strongly for the importance of this receptor in functions of glutamate neurotransmission. RNA blot analysis showed abundant expression of mRNA whose size is about 4.5 and 4.8 kilonucleotides. The human gene encoding the NMDAR1 subunit has been mapped to chromosome 9q34.3 by the analyses of blot hybridization of a DNA panel of human/hamster somatic cell hybrids and fluorescence in situ hybridization of human chromosomes.
Volume 268(5)
Pages 3728-33
Published 1993-2-15
PMID 7679115
MeSH 2-Amino-5-phosphonovalerate / pharmacology Amino Acid Sequence Animals Base Sequence Brain / metabolism* Chromosome Mapping Chromosomes, Human, Pair 9* Cricetinae DNA / genetics DNA / isolation & purification Dizocilpine Maleate / pharmacology Humans Hybrid Cells Karyotyping Kynurenic Acid / pharmacology Macromolecular Substances Magnesium / pharmacology Molecular Sequence Data N-Methylaspartate / pharmacology* Oocytes / drug effects Oocytes / physiology Poly A / genetics Poly A / isolation & purification RNA / genetics RNA / isolation & purification RNA, Messenger Receptors, N-Methyl-D-Aspartate / drug effects Receptors, N-Methyl-D-Aspartate / genetics* Receptors, N-Methyl-D-Aspartate / physiology Temporal Lobe / metabolism* Xenopus Zinc / pharmacology
IF 4.106
Times Cited 100
DNA material phNR 1 (RDB13203)