RRC ID 48110
Author Kitano J, Yamazaki Y, Kimura K, Masukado T, Nakajima Y, Nakanishi S.
Title Tamalin is a scaffold protein that interacts with multiple neuronal proteins in distinct modes of protein-protein association.
Journal J Biol Chem
Abstract Tamalin is a scaffold protein that comprises multiple protein-interacting domains, including a 95-kDa postsynaptic density protein (PSD-95)/discs-large/ZO-1 (PDZ) domain, a leucine-zipper region, and a carboxyl-terminal PDZ binding motif. Tamalin forms a complex with metabotropic glutamate receptors and guanine nucleotide exchange factor cytohesins and promotes intracellular trafficking and cell surface expression of group 1 metabotropic glutamate receptors. In the present study, using several different approaches we have shown that tamalin interacts with multiple neuronal proteins through its distinct protein-binding domains. The PDZ domain of tamalin binds to the PDZ binding motifs of SAP90/PSD-95-associated protein and tamalin itself, whereas the PDZ binding motif of tamalin is capable of interacting with the PDZ domain of S-SCAM. In addition, tamalin forms a complex with PSD-95 and Mint2/X11beta/X11L by mechanisms different from the PDZ-mediated interaction. Tamalin has the ability to assemble with these proteins in vivo; their protein complex with tamalin was verified by coimmunoprecipitation of rat brain lysates. Interestingly, the distinct protein-interacting domains of tamalin are evolutionarily conserved, and mRNA expression is developmentally up-regulated at the postnatal period. The results indicate that tamalin exists as a key element that forms a protein complex with multiple postsynaptic and protein-trafficking scaffold proteins.
Volume 278(17)
Pages 14762-8
Published 2003-4-25
DOI 10.1074/jbc.M300184200
PII S0021-9258(19)30269-8
PMID 12586822
MeSH Adaptor Proteins, Signal Transducing Amino Acid Sequence Animals Cadherins* Carrier Proteins / chemistry Carrier Proteins / metabolism* Carrier Proteins / physiology Conserved Sequence Disks Large Homolog 4 Protein Guanylate Kinases Intracellular Signaling Peptides and Proteins Membrane Proteins Nerve Tissue Proteins / chemistry Nerve Tissue Proteins / metabolism* Neurons / chemistry Protein Binding Protein Structure, Tertiary Rats SAP90-PSD95 Associated Proteins Sequence Alignment
IF 4.238
Times Cited 53
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
DNA material Tamalin pcDNA3.1 (RDB13213)