RRC ID 48166
著者 Tanaka S, Yamamoto K, Yamada K, Furuya K, Uyeno Y.
タイトル Relationship of Enhanced Butyrate Production by Colonic Butyrate-Producing Bacteria to Immunomodulatory Effects in Normal Mice Fed an Insoluble Fraction of Brassica rapa L.
ジャーナル Appl Environ Microbiol
Abstract This study was performed to determine the effects of feeding a fiber-rich fraction of Brassica vegetables on the immune response through changes in enteric bacteria and short-chain fatty acid (SCFA) production in normal mice. The boiled-water-insoluble fraction of Brassica rapa L. (nozawana), which consists mainly of dietary fiber, was chosen as a test material. A total of 31 male C57BL/6J mice were divided into two groups and housed in a specific-pathogen-free facility. The animals were fed either a control diet or the control diet plus the insoluble B. rapa L. fraction for 2 weeks and sacrificed to determine microbiological and SCFA profiles in lower-gut samples and immunological molecules. rRNA-based quantification indicated that the relative population of Bacteroidetes was markedly lower in the colon samples of the insoluble B. rapa L. fraction-fed group than that in the controls. Populations of the Eubacterium rectale group and Faecalibacterium prausnitzii, both of which are representative butyrate-producing bacteria, doubled after 2 weeks of fraction intake, accompanying a marginal increase in the proportion of colonic butyrate. In addition, feeding with the fraction significantly increased levels of the anti-inflammatory cytokine interleukin-10 (IL-10) and tended to increase splenic regulatory T cell numbers but significantly reduced the population of cells expressing activation markers. We demonstrated that inclusion of the boiled-water-insoluble fraction of B. rapa L. can alter the composition of the gut microbiota to decrease the numbers of Bacteroidetes and to increase the numbers of butyrate-producing bacteria, either of which may be involved in the observed shift in the production of splenic IL-10.
巻・号 82(9)
ページ 2693-2699
公開日 2016-5-1
DOI 10.1128/AEM.03343-15
PII AEM.03343-15
PMID 26921420
PMC PMC4836433
MeSH Animals Bacteria / drug effects Bacteria / immunology Bacteria / isolation & purification Bacteria / metabolism* Bacteroidetes / drug effects Bacteroidetes / isolation & purification Bacteroidetes / metabolism Brassica rapa* Butyric Acid / metabolism* Colon / drug effects Colon / immunology* Colon / microbiology* Dietary Fiber / administration & dosage* Dietary Supplements Eubacterium / drug effects Eubacterium / isolation & purification Eubacterium / metabolism Faecalibacterium / drug effects Faecalibacterium / isolation & purification Faecalibacterium / metabolism Fatty Acids, Volatile / biosynthesis Feces / microbiology Interleukin-10 / immunology Male Mice Mice, Inbred C57BL T-Lymphocytes / immunology
IF 4.016
引用数 13
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 17581 JCM 14654