論文 - 詳細
| RRC ID | 48166 |
|---|---|
| 著者 | Tanaka S, Yamamoto K, Yamada K, Furuya K, Uyeno Y. |
| タイトル | Relationship of Enhanced Butyrate Production by Colonic Butyrate-Producing Bacteria to Immunomodulatory Effects in Normal Mice Fed an Insoluble Fraction of Brassica rapa L. |
| ジャーナル | Appl Environ Microbiol |
| Abstract |
This study was performed to determine the effects of feeding a fiber-rich fraction of Brassica vegetables on the immune response through changes in enteric bacteria and short-chain fatty acid (SCFA) production in normal mice. The boiled-water-insoluble fraction of Brassica rapa L. (nozawana), which consists mainly of dietary fiber, was chosen as a test material. A total of 31 male C57BL/6J mice were divided into two groups and housed in a specific-pathogen-free facility. The animals were fed either a control diet or the control diet plus the insoluble B. rapa L. fraction for 2 weeks and sacrificed to determine microbiological and SCFA profiles in lower-gut samples and immunological molecules. rRNA-based quantification indicated that the relative population of Bacteroidetes was markedly lower in the colon samples of the insoluble B. rapa L. fraction-fed group than that in the controls. Populations of the Eubacterium rectale group and Faecalibacterium prausnitzii, both of which are representative butyrate-producing bacteria, doubled after 2 weeks of fraction intake, accompanying a marginal increase in the proportion of colonic butyrate. In addition, feeding with the fraction significantly increased levels of the anti-inflammatory cytokine interleukin-10 (IL-10) and tended to increase splenic regulatory T cell numbers but significantly reduced the population of cells expressing activation markers. We demonstrated that inclusion of the boiled-water-insoluble fraction of B. rapa L. can alter the composition of the gut microbiota to decrease the numbers of Bacteroidetes and to increase the numbers of butyrate-producing bacteria, either of which may be involved in the observed shift in the production of splenic IL-10. |
| 巻・号 | 82(9) |
| ページ | 2693-2699 |
| 公開日 | 2016-5-1 |
| DOI | 10.1128/AEM.03343-15 |
| PII | AEM.03343-15 |
| PMID | 26921420 |
| PMC | PMC4836433 |
| MeSH | Animals Bacteria / drug effects Bacteria / immunology Bacteria / isolation & purification Bacteria / metabolism* Bacteroidetes / drug effects Bacteroidetes / isolation & purification Bacteroidetes / metabolism Brassica rapa* Butyric Acid / metabolism* Colon / drug effects Colon / immunology* Colon / microbiology* Dietary Fiber / administration & dosage* Dietary Supplements Eubacterium / drug effects Eubacterium / isolation & purification Eubacterium / metabolism Faecalibacterium / drug effects Faecalibacterium / isolation & purification Faecalibacterium / metabolism Fatty Acids, Volatile / biosynthesis Feces / microbiology Interleukin-10 / immunology Male Mice Mice, Inbred C57BL T-Lymphocytes / immunology |
| IF | 4.016 |
| 引用数 | 13 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 17581 JCM 14654 |