RRC ID 48174
著者 Ji SQ, Wang B, Lu M, Li FL.
タイトル Defluviitalea phaphyphila sp. nov., a Novel Thermophilic Bacterium That Degrades Brown Algae.
ジャーナル Appl Environ Microbiol
Abstract Brown algae are one of the largest groups of oceanic primary producers for CO2 removal and carbon sinks for coastal regions. However, the mechanism for brown alga assimilation remains largely unknown in thermophilic microorganisms. In this work, a thermophilic alginolytic community was enriched from coastal sediment, from which an obligate anaerobic and thermophilic bacterial strain, designated Alg1, was isolated. Alg1 shared a 16S rRNA gene identity of 94.6% with Defluviitalea saccharophila LIND6LT2(T). Phenotypic, chemotaxonomic, and phylogenetic studies suggested strain Alg1 represented a novel species of the genus Defluviitalea, for which the name Defluviitalea phaphyphila sp. nov. is proposed. Alg1 exhibited an intriguing ability to convert carbohydrates of brown algae, including alginate, laminarin, and mannitol, to ethanol and acetic acid. Three gene clusters participating in this process were predicted to be in the genome, and candidate enzymes were successfully expressed, purified, and characterized. Six alginate lyases were demonstrated to synergistically deconstruct alginate into unsaturated monosaccharide, followed by one uronic acid reductase and two 2-keto-3-deoxy-d-gluconate (KDG) kinases to produce pyruvate. A nonclassical mannitol 1-phosphate dehydrogenase, catalyzing D-mannitol 1-phosphate to fructose 1-phosphate in the presence of NAD(+), and one laminarase also were disclosed. This work revealed that a thermophilic brown alga-decomposing system containing numerous novel thermophilic alginate lyases and a unique mannitol 1-phosphate dehydrogenase was adopted by the natural ethanologenic strain Alg1 during the process of evolution in hostile habitats.
巻・号 82(3)
ページ 868-77
公開日 2016-2-1
DOI 10.1128/AEM.03297-15
PII AEM.03297-15
PMID 26590273
PMC PMC4725288
MeSH Acetic Acid / metabolism Alginates / metabolism Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics* DNA, Ribosomal / genetics Ethanol / metabolism Genome, Bacterial Geologic Sediments / microbiology* Glucans / metabolism Glucuronic Acid / metabolism Gram-Positive Bacteria / classification* Gram-Positive Bacteria / genetics Gram-Positive Bacteria / isolation & purification* Gram-Positive Bacteria / metabolism* Hexuronic Acids / metabolism Mannitol / metabolism Multigene Family Phaeophyta / metabolism* Phylogeny Pyruvic Acid / metabolism RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA
IF 4.016
引用数 15
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 30481