| RRC ID |
48197
|
| Author |
Bharathiraja B, Ranjith Kumar R, PraveenKumar R, Chakravarthy M, Yogendran D, Jayamuthunagai J.
|
| Title |
Biodiesel production from different algal oil using immobilized pure lipase and tailor made rPichia pastoris with Cal A and Cal B genes.
|
| Journal |
Bioresour Technol
|
| Abstract |
In this investigation, oil extraction was performed in marine macroalgae Gracilaria edulis, Enteromorpha compressa and Ulva lactuca. The algal biomass was characterized by Scanning Electron Microscopy and Fourier Transform-Infra Red Spectroscopy. Six different pre-treatment methods were carried out to evaluate the best method for maximum oil extraction. Optimization of extraction parameters were performed and high oil yield was obtained at temperature 55°C, time 150min, particle size 0.10mm, solvent-to-solid ratio 6:1 and agitation rate 500rpm. After optimization, 9.5%, 12.18% and 10.50 (g/g) of oil extraction yield was achieved from the respective algal biomass. The rate constant for extraction was obtained as first order kinetics, by differential method. Stable intracellular Cal A and Cal B lipase producing recombinant Pichia pastoris was constructed and used as biocatalyst for biodiesel production. Comparative analysis of lipase activity and biodiesel yield was made with immobilized Candida antarctica lipase.
|
| Volume |
213
|
| Pages |
69-78
|
| Published |
2016-8-1
|
| DOI |
10.1016/j.biortech.2016.02.041
|
| PII |
S0960-8524(16)30167-5
|
| PMID |
26906444
|
| MeSH |
Biocatalysis
Biofuels* / analysis
Biotechnology / methods*
Cooking
Enzymes, Immobilized / metabolism*
Esterification
Fatty Acids / analysis
Kinetics
Lipase / metabolism*
Oils / metabolism*
Pichia / metabolism*
Seaweed / metabolism*
Spectroscopy, Fourier Transform Infrared
Ulva / metabolism
Waste Products
|
| IF |
7.539
|
| Times Cited |
12
|
|
WOS Category
|
AGRICULTURAL ENGINEERING
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
ENERGY & FUELS
|
| Resource |
| General Microbes |
JCM 10318 |
