Reference - Detail
| RRC ID | 4819 |
|---|---|
| Author | Timmers J, Vernhettes S, Desprez T, Vincken JP, Visser RG, Trindade LM. |
| Title | Interactions between membrane-bound cellulose synthases involved in the synthesis of the secondary cell wall. |
| Journal | FEBS Lett |
| Abstract |
It has not yet been reported how the secondary CESA (cellulose synthase) proteins are organized in the rosette structure. A membrane-based yeast two-hybrid (MbYTH) approach was used to analyze the interactions between the CESA proteins involved in secondary cell wall synthesis of Arabidopsis and the findings were confirmed in planta by bimolecular fluorescence complementation (BiFC) assay. Results indicated that although all CESA proteins can interact with each other, only CESA4 is able to form homodimers. A model is proposed for the secondary rosette structure. The RING-motif proved not to be essential for the interaction between the CESA proteins. |
| Volume | 583(6) |
| Pages | 978-82 |
| Published | 2009-3-18 |
| DOI | 10.1016/j.febslet.2009.02.035 |
| PII | S0014-5793(09)00149-5 |
| PMID | 19258017 |
| MeSH | Amino Acid Sequence Amino Acid Substitution / physiology Cell Wall / metabolism* Glucosyltransferases / metabolism* Glucosyltransferases / physiology* Membrane Proteins / metabolism Molecular Sequence Data Plant Proteins / metabolism Plant Proteins / physiology Plants / metabolism Protein Binding Protein Multimerization / physiology RING Finger Domains / physiology Sequence Homology, Amino Acid Two-Hybrid System Techniques |
| IF | 3.057 |
| Times Cited | 46 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pda13285 pda09751 pda08626 |