RRC ID 48206
Author Zarrinfar H, Kaboli S, Dolatabadi S, Mohammadi R.
Title Rapid detection of Candida species in bronchoalveolar lavage fluid from patients with pulmonary symptoms.
Journal Braz J Microbiol
Abstract Candida species, especially C. albicans, are commensals on human mucosal surfaces, but are increasingly becoming one of the important invasive pathogens as seen by a rise in its prevalence in immunocompromised patients and in antibiotic consumption. Thus, an accurate identification of Candida species in patients with pulmonary symptoms can provide important information for effective treatment. A total of 75 clinical isolates of Candida species were obtained from the bronchoalveolar lavage fluid of both immunocompromised and immunocompetent patients with pulmonary symptoms. Candida cultures were identified based on nuclear ribosomal Internal Transcribed Spacer (ITS1-ITS2 rDNA) sequence analysis by polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP). Molecular identification indicated that the isolates belonged predominantly to C. albicans (52%), followed by C. tropicalis (24%), C. glabrata (14.7%), C. krusei (5.3%), C. parapsilosis (1.3%), C. kefyr (1.3%) and C. guilliermondii (1.3%). Given the increasing complexity of disease profiles and their management regimens in diverse patients, rapid and accurate identification of Candida species can lead to timely and appropriate antifungal therapy.
Volume 47(1)
Pages 172-6
Published 2016-1-1
DOI 10.1016/j.bjm.2015.02.001
PII S1517-8382(15)00030-1
PMID 26887241
PMC PMC4822774
MeSH Bronchoalveolar Lavage Fluid / microbiology* Candida / classification Candida / genetics Candida / isolation & purification* Candidiasis / diagnosis* DNA, Fungal / chemistry DNA, Fungal / genetics DNA, Ribosomal Spacer / chemistry DNA, Ribosomal Spacer / genetics Humans Lung Diseases, Fungal / diagnosis* Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Sequence Analysis, DNA Time Factors
IF 2.428
Times Cited 11
WOS Category MICROBIOLOGY
Resource
General Microbes JCM 1439 JCM 2309 JCM 9555 JCM 1607 JCM 1619 JCM 9567