RRC ID |
48206
|
Author |
Zarrinfar H, Kaboli S, Dolatabadi S, Mohammadi R.
|
Title |
Rapid detection of Candida species in bronchoalveolar lavage fluid from patients with pulmonary symptoms.
|
Journal |
Braz J Microbiol
|
Abstract |
Candida species, especially C. albicans, are commensals on human mucosal surfaces, but are increasingly becoming one of the important invasive pathogens as seen by a rise in its prevalence in immunocompromised patients and in antibiotic consumption. Thus, an accurate identification of Candida species in patients with pulmonary symptoms can provide important information for effective treatment. A total of 75 clinical isolates of Candida species were obtained from the bronchoalveolar lavage fluid of both immunocompromised and immunocompetent patients with pulmonary symptoms. Candida cultures were identified based on nuclear ribosomal Internal Transcribed Spacer (ITS1-ITS2 rDNA) sequence analysis by polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP). Molecular identification indicated that the isolates belonged predominantly to C. albicans (52%), followed by C. tropicalis (24%), C. glabrata (14.7%), C. krusei (5.3%), C. parapsilosis (1.3%), C. kefyr (1.3%) and C. guilliermondii (1.3%). Given the increasing complexity of disease profiles and their management regimens in diverse patients, rapid and accurate identification of Candida species can lead to timely and appropriate antifungal therapy.
|
Volume |
47(1)
|
Pages |
172-6
|
Published |
2016-1-1
|
DOI |
10.1016/j.bjm.2015.02.001
|
PII |
S1517-8382(15)00030-1
|
PMID |
26887241
|
PMC |
PMC4822774
|
MeSH |
Bronchoalveolar Lavage Fluid / microbiology*
Candida / classification
Candida / genetics
Candida / isolation & purification*
Candidiasis / diagnosis*
DNA, Fungal / chemistry
DNA, Fungal / genetics
DNA, Ribosomal Spacer / chemistry
DNA, Ribosomal Spacer / genetics
Humans
Lung Diseases, Fungal / diagnosis*
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Sequence Analysis, DNA
Time Factors
|
IF |
2.428
|
Times Cited |
11
|
WOS Category
|
MICROBIOLOGY
|
Resource |
General Microbes |
JCM 1439
JCM 2309
JCM 9555
JCM 1607
JCM 1619
JCM 9567 |