論文 - 詳細
| RRC ID | 48228 |
|---|---|
| 著者 | Maeda K, Tanaka A, Sugiura R, Koshino H, Tokai T, Sato M, Nakajima Y, Tanahashi Y, Kanamaru K, Kobayashi T, Nishiuchi T, Fujimura M, Takahashi-Ando N, Kimura M. |
| タイトル | Hydroxylations of trichothecene rings in the biosynthesis of Fusarium trichothecenes: evolution of alternative pathways in the nivalenol chemotype. |
| ジャーナル | Environ Microbiol |
| Abstract |
Fusarium sporotrichioides genes FsTri11, FsTri13, and FsTri1 encode cytochrome P450 monooxygenases (CYPs) responsible for hydroxylations at C-15, C-4, and C-8 of the trichothecene skeleton, respectively. However, the corresponding genes of nivalenol (NIV)-chemotype Fusarium graminearum remain to be functionally elucidated. In this study, we characterized the roles of these CYPs in NIV biosynthesis. Analyses of the metabolites of the F. graminearum Fgtri11- mutant, a disruptant of FgTri11 encoding isotrichodermin (ITD) C-15 hydroxylase, revealed a small amount of NIV-type trichothecenes suggesting that an alternative C-15 hydroxylase partially complemented FgTRI11p. In contrast, the C-7/C-8 hydroxylations depended solely on FgTRI1p, as suggested by the metabolite profiles of the Fgtri11- Fgtri1- double gene disruptant. Disruption of FgTri1 in both the wild-type and Fgtri13- mutant backgrounds revealed that FgTRI13p exhibits marginal activity toward calonectrin (CAL) and that it was the only C-4 hydroxylase. In addition, feeding experiments demonstrated that the C-4 hydroxylation of a 7-hydroxytrichothecene lacking C-8 ketone was extremely limited. The marginal activity of FgTRI13p toward CAL was advantageous for the C-7/C-8 hydroxylation steps in NIV biosynthesis, as transformation of a C-4 oxygenated trichothecene lacking C-7/C-8 modifications into NIV-type trichothecenes was quite inefficient. The significance of hydroxylation steps in the evolution of Fusarium trichothecenes is discussed. |
| 巻・号 | 18(11) |
| ページ | 3798-3811 |
| 公開日 | 2016-11-1 |
| DOI | 10.1111/1462-2920.13338 |
| PMID | 27120196 |
| MeSH | Biological Evolution Cytochrome P-450 Enzyme System / genetics Cytochrome P-450 Enzyme System / metabolism Fungal Proteins / genetics Fungal Proteins / metabolism Fusarium / chemistry Fusarium / enzymology Fusarium / genetics Fusarium / metabolism* Hydroxylation Trichothecenes / biosynthesis* Trichothecenes / chemistry Trichothecenes / metabolism |
| IF | 4.933 |
| 引用数 | 8 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 9873 |