RRC ID 48239
著者 Iwatani S, Ishibashi N, Flores FP, Zendo T, Nakayama J, Sonomoto K.
タイトル LnqR, a TetR-family transcriptional regulator, positively regulates lacticin Q production in Lactococcus lactis QU 5.
ジャーナル FEMS Microbiol Lett
Abstract Lacticin Q is an unmodified leaderless bacteriocin produced by Lactococcus lactis QU 5. It has been revealed that the production and self-immunity of lacticin Q are facilitated by a gene cluster lnqQBCDEF The gene for a putative TetR-family transcriptional regulator, termed lnqR, was found nearby the lnqQBCDEF cluster, but its involvement in lacticin Q biosynthesis remained unknown. In this study, we created an LnqR-overexpressing QU 5 recombinant by using lactococcal constitutive promoter P32 The recombinant QU 5 showed enhanced production of and self-immunity to lacticin Q. RT-PCR analysis has revealed that an overexpression of LnqR increases the amounts of lnqQBCDEF transcripts, and these six genes are transcribed as an operon in a single transcriptional unit. Interestingly, LnqR expression and thus lacticin Q production by L. lactis QU 5 was found temperature dependent, while LnzR, an LnqR-homologue, in L. lactis QU 14 was expressed in a similar but not identical manner to LnqR, resulting in dissimilar bacteriocin productivities by these strains. This report demonstrates LnqR as the first TetR-family transcriptional regulator involved in LAB bacteriocin biosynthesis and that, as an exceptional case of TetR-family regulators, LnqR positively regulates the transcription of these biosynthetic genes.
巻・号 363(18)
公開日 2016-9-1
DOI 10.1093/femsle/fnw200
PII fnw200
PMID 27549303
MeSH Bacteriocins / biosynthesis* Gene Expression Regulation, Bacterial* Genes, Bacterial* Lactococcus lactis / genetics* Lactococcus lactis / metabolism Multigene Family Operon Promoter Regions, Genetic Real-Time Polymerase Chain Reaction
IF 1.987
引用数 4
リソース情報
一般微生物 JCM 2257