RRC ID 4828
Author Stålberg K, Ståhl U, Stymne S, Ohlrogge J.
Title Characterization of two Arabidopsis thaliana acyltransferases with preference for lysophosphatidylethanolamine.
Journal BMC Plant Biol.
Abstract BACKGROUND:Two previously uncharacterized Arabidopsis genes that encode proteins with acyltransferase PlsC regions were selected for study based on their sequence similarity to a recently identified lung lysophosphatidylcholine acyltransferase (LPCAT). To identify their substrate specificity and biochemical properties, the two Arabidopsis acyltransferases, designated AtLPEAT1, (At1g80950), and AtLPEAT2 (At2g45670) were expressed in yeast knockout lines ale1 and slc1 that are deficient in microsomal lysophosphatidyl acyltransferase activities.
RESULTS:Expression of AtLPEAT1 in the yeast knockout ale1 background exhibited strong acylation activity of lysophosphatidylethanolamine (LPE) and lysophosphatidate (LPA) with lower activity on lysophosphatidylcholine (LPC) and lysophosphatidylserine (LPS). AtLPEAT2 had specificities in the order of LPE > LPC > LPS and had no or very low activity with LPA. Both acyltransferases preferred 18:1-LPE over 16:0-LPE as acceptor and preferred palmitoyl-CoA as acyl donor in combination with 18:1-LPE. Both acyltransferases showed no or minor responses to Ca2+, despite the presence of a calcium binding EF-hand region in AtLPEAT2. AtLPEAT1 was more active at basic pH while AtLPEAT2 was equally active between pH 6.0 - 9.0.
CONCLUSION:This study represents the first description of plant acyltransferases with a preference for LPE. In conclusion it is suggested that the two AtLPEATs, with their different biochemical and expression properties, have different roles in membrane metabolism/homoeostasis.
Volume 9
Pages 60
Published 2009-5-16
DOI 10.1186/1471-2229-9-60
PII 1471-2229-9-60
PMID 19445718
PMC PMC2690597
MeSH Acylation Acyltransferases / genetics Acyltransferases / metabolism* Amino Acid Sequence Arabidopsis / enzymology Arabidopsis / genetics* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* DNA, Complementary / genetics Lysophospholipids / metabolism* Molecular Sequence Data Substrate Specificity
IF 3.93
Times Cited 20
WOS Category PLANT SCIENCES
Resource
Arabidopsis / Cultured plant cells, genes pda09299 pda10945