RRC ID 48437
著者 Taguchi Y, Fujinami D, Kohda D.
タイトル Comparative Analysis of Archaeal Lipid-linked Oligosaccharides That Serve as Oligosaccharide Donors for Asn Glycosylation.
ジャーナル J Biol Chem
Abstract The glycosylation of asparagine residues is the predominant protein modification in all three domains of life. An oligosaccharide chain is preassembled on a lipid-phospho carrier and transferred onto asparagine residues by the action of a membrane-bound enzyme, oligosaccharyltransferase. The oligosaccharide donor for the oligosaccharyl transfer reaction is dolichol-diphosphate-oligosaccharide in Eukaryota and polyprenol-diphosphate-oligosaccharide in Eubacteria. The donor in some archaeal species was reportedly dolichol-monophosphate-oligosaccharide. Thus, the difference in the number of phosphate groups aroused interest in whether the use of the dolichol-monophosphate type donors is widespread in the domain Archaea. Currently, all of the archaeal species with identified oligosaccharide donors have belonged to the phylum Euryarchaeota. Here, we analyzed the donor structures of two species belonging to the phylum Crenarchaeota, Pyrobaculum calidifontis and Sulfolobus solfataricus, in addition to two species from the Euryarchaeota, Pyrococcus furiosus and Archaeoglobus fulgidus The electrospray ionization tandem mass spectrometry analyses confirmed that the two euryarchaeal oligosaccharide donors were the dolichol-monophosphate type and newly revealed that the two crenarchaeal oligosaccharide donors were the dolichol-diphosphate type. This novel finding is consistent with the hypothesis that the ancestor of Eukaryota is rooted within the TACK (Thaum-, Aig-, Cren-, and Korarchaeota) superphylum, which includes Crenarchaea. Our comprehensive study also revealed that one archaeal species could contain two distinct oligosaccharide donors for the oligosaccharyl transfer reaction. The A. fulgidus cells contained two oligosaccharide donors bearing oligosaccharide moieties with different backbone structures, and the S. solfataricus cells contained two oligosaccharide donors bearing stereochemically different dolichol chains.
巻・号 291(21)
ページ 11042-54
公開日 2016-5-20
DOI 10.1074/jbc.M115.713156
PII S0021-9258(20)40848-8
PMID 27015803
PMC PMC4900254
MeSH Archaea / classification Archaea / metabolism* Archaeal Proteins / metabolism Archaeoglobus fulgidus / metabolism Asparagine / chemistry Asparagine / metabolism* Glycosylation Hexosyltransferases / metabolism Membrane Proteins / metabolism Molecular Structure Polyisoprenyl Phosphate Oligosaccharides / chemistry* Polyisoprenyl Phosphate Oligosaccharides / metabolism* Pyrobaculum / metabolism Pyrococcus furiosus / metabolism Spectrometry, Mass, Electrospray Ionization Sulfolobus solfataricus / metabolism Tandem Mass Spectrometry
IF 4.238
引用数 26
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
一般微生物 JCM 11548