論文 - 詳細
| RRC ID | 48442 |
|---|---|
| 著者 | Ng LM, Sudesh K. |
| タイトル | Identification of a new polyhydroxyalkanoate (PHA) producer Aquitalea sp. USM4 (JCM 19919) and characterization of its PHA synthase. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
Aquitalea sp. USM4 (JCM 19919) was isolated from a freshwater sample at Lata Iskandar Waterfall in Perak, Malaysia. It is a rod-shaped, gram-negative bacterium with high sequence identity (99%) to Aquitalea magnusonii based on 16S rRNA gene analysis. Aquitalea sp. USM4 also possessed a PHA synthase gene (phaC), which had amino acid sequence identity of 77-78% to the PHA synthase of Chromobacterium violaceum ATCC12472 and Pseudogulbenkiania sp. NH8B. PHA biosynthesis results showed that wild-type Aquitalea sp. USM4 was able to accumulate up to 1.5 g/L of poly(3-hydroxybutyrate), [P(3HB)]. The heterologous expression of the PHA synthase gene of Aquitalea sp. USM4 (phaCAq) in Cupriavidus necator PHB-4 had resulted in PHA accumulation up to 3.2 g/L of P(3HB). It was further confirmed by 1H nuclear magnetic resonance (NMR) analysis that Aquitalea sp. USM4 and C. necator PHB-4 transformant were able to produce PHA containing 3-hydroxyvalerate (3HV), 4-hydroxybutyrate (4HB) and 3-hydroxy-4-methylvalerate (3H4MV) monomers from suitable precursor substrates. Interestingly, relatively high PHA synthase activity of 863 U/g and 1402 U/g were determined in wild-type Aquitalea sp. USM4 and C. necator PHB-4 transformant respectively. This is the first report on the member of genus Aquitalea as a new PHA producer as well as in vitro and in vivo characterization of a novel PHA synthase from Aquitalea sp. USM4. |
| 巻・号 | 122(5) |
| ページ | 550-557 |
| 公開日 | 2016-11-1 |
| DOI | 10.1016/j.jbiosc.2016.03.024 |
| PII | S1389-1723(16)30046-9 |
| PMID | 27132174 |
| MeSH | Acyltransferases / genetics* Acyltransferases / isolation & purification Acyltransferases / metabolism* Amino Acid Sequence Chromobacterium / enzymology* Chromobacterium / genetics Chromobacterium / isolation & purification* Chromobacterium / metabolism Cloning, Molecular Cupriavidus necator / enzymology Cupriavidus necator / genetics Cupriavidus necator / metabolism Fresh Water / microbiology Pentanoic Acids / metabolism Phylogeny Polyesters / metabolism Polyhydroxyalkanoates / biosynthesis* RNA, Ribosomal, 16S / metabolism Sequence Analysis, DNA |
| IF | 2.366 |
| 引用数 | 9 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 一般微生物 | JCM 19919 |