RRC ID 48451
Author Toshimitsu T, Mochizuki J, Ikegami S, Itou H.
Title Identification of a Lactobacillus plantarum strain that ameliorates chronic inflammation and metabolic disorders in obese and type 2 diabetic mice.
Journal J Dairy Sci
Abstract In this study, we identified a strain of lactic acid bacteria (LAB) that induces high levels of IL-10 production by immune cells, and evaluated the ability of the strain to suppress chronic inflammation and ameliorate metabolic disorders in in vitro and in vivo models. Among a collection of LAB strains, Lactobacillus plantarum strain OLL2712 (OLL2712) induced the highest levels of IL-10 production in mouse-derived dendritic cells and peritoneal macrophages. The anti-inflammatory effects of this strain were evaluated using a co-culture system comprising RAW 264.7 and 3T3-L1 cells. We also administered heat-killed OLL2712 to obese and type 2 diabetic KKAy mice for 3 wk to evaluate the in vivo effects of the strain. The OLL2712 significantly decreased the production of proinflammatory cytokines in vitro. Likewise, the administration of OLL2712 significantly suppressed proinflammatory cytokine levels in both the visceral adipose tissue and the serum of KKAy mice, and reduced serum triglyceride concentrations. The strain also alleviated oxidative stress and adrenaline levels in the serum of KKAy mice. On the other hand, Lactobacillus gasseri strain MEP222804 (a moderate IL-10 inducer) did not ameliorate the systemic inflammation and hyperlipidemia in KKAy mice. Our results suggest that treatment with strong IL-10-inducing LAB has the potential to ameliorate metabolic disorders by suppressing chronic inflammation in the host animal.
Volume 99(2)
Pages 933-946
Published 2016-2-1
DOI 10.3168/jds.2015-9916
PII S0022-0302(15)00885-1
PMID 26686731
MeSH 3T3-L1 Cells Animals Coculture Techniques Cytokines / biosynthesis Dendritic Cells / metabolism Diabetes Mellitus, Type 2 / complications* Feces / microbiology Female Humans Inflammation / therapy* Interleukin-10 / biosynthesis Lactobacillus plantarum / isolation & purification* Lactobacillus plantarum / physiology* Macrophages, Peritoneal / metabolism Metabolic Diseases / therapy* Mice Mice, Inbred BALB C Obesity / complications* RAW 264.7 Cells
IF 3.333
Times Cited 16
WOS Category AGRICULTURE, DAIRY & ANIMAL SCIENCE FOOD SCIENCE & TECHNOLOGY
Resource
General Microbes JCM 1131  JCM 1149