論文 - 詳細
| RRC ID | 48452 |
|---|---|
| 著者 | Vartoukian SR, Moazzez RV, Paster BJ, Dewhirst FE, Wade WG. |
| タイトル | First Cultivation of Health-Associated Tannerella sp. HOT-286 (BU063). |
| ジャーナル | J Dent Res |
| Abstract |
Despite significant advances in recent years in culture-independent molecular microbiology methods, the detailed study of individual bacterial species still relies on having pure cultures in the laboratory. Yet, more than a third of the approximately 700 bacterial taxa found in the human oral cavity are as yet uncultivated in vitro. One such taxon, Tannerella sp. HOT-286 (phylotype BU063), is the focus of much interest since it is associated with periodontal health, while Tannerella forsythia, its closest phylogenetic neighbor, is strongly associated with periodontal disease. HOT-286, however, has remained uncultivated despite the efforts of several research groups, spanning over a decade. The aim of this study was to cultivate Tannerella sp. HOT-286. A heavily diluted sample of subgingival plaque was inoculated onto culture plates supplemented with siderophores (pyoverdines-Fe complex or desferricoprogen) or a neat plaque suspension. After 8 d of anaerobic incubation, microcolonies and colonies showing satellitism were passaged onto fresh culture plates cross-streaked with potential helper strains or onto cellulose-acetate membranes placed over lawn cultures of helper strains. Subcultured colonies were identified by 16S rRNA gene sequencing, and purity was confirmed by sequencing 20 clones per library prepared from a single colony. Three colonies of interest (derived from pyoverdines- and plaque-supplemented plates) were identified as Tannerella sp. HOT-286. The isolates were found to be incapable of independent growth, requiring helpers such as Propionibacterium acnes and Prevotella intermedia for stimulation, with best growth on membranes over "helper" lawns. A representative isolate was subjected to phenotypic characterization and found to produce a range of glycosidic and proteolytic enzymes. Further comparison of this novel "periodontal health-associated" taxon with T. forsythia will be valuable in investigating virulence factors of the latter and possible health benefits of the former. |
| 巻・号 | 95(11) |
| ページ | 1308-13 |
| 公開日 | 2016-10-1 |
| DOI | 10.1177/0022034516651078 |
| PII | 0022034516651078 |
| PMID | 27193146 |
| PMC | PMC5076754 |
| MeSH | Bacteriological Techniques / methods Culture Media Dental Plaque / microbiology Female Humans Middle Aged Periodontal Diseases / microbiology Tannerella forsythia / growth & development* Tannerella forsythia / pathogenicity |
| IF | 4.914 |
| 引用数 | 9 |
| WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 31301 JCM 31302 JCM 31303 |