| RRC ID |
48455
|
| 著者 |
Také A, Nakashima T, Inahashi Y, Shiomi K, Takahashi Y, Ōmura S, Matsumoto A.
|
| タイトル |
Analyses of the cell-wall peptidoglycan structures in three genera Micromonospora, Catenuloplanes, and Couchioplanes belonging to the family Micromonosporaceae by derivatization with FDLA and PMP using LC/MS.
|
| ジャーナル |
J Gen Appl Microbiol
|
| Abstract |
It is the major characteristic of the cell-wall peptidoglycan structure in members of the family Micromonosporaceae that N-acetylmuramic acid (MurNAc) of glycan strand is replaced with N-glycolylmuramic acid (MurNGlyc). Consequently, it is difficult to use enzymatic methods for their peptidoglycan analyses. We therefore developed analysis method of peptidoglycan without using cell wall lytic enzymes as example to take the 3 genera, Micromonospora, Catenuloplanes, and Couchioplanes belonging to the family Micromonosporaceae, and their peptidoglycans were partially hydrolyzed with 4 M HCl at 60°C for 16 h followed by derivatization with N(α)-(5-fluoro-2,4-dinitrophenyl)-D-leucinamide (FDLA) or 1-phenyl-3-methyl-5-pyrazolone (PMP) and LC/MS analysis. Peptidoglycan of the genus Micromonospora consisted of a MurNGlyc-Gly-D-Glu-meso-diaminopimelyl (DAP)-D-Ala peptide stem and direct linkage between D-Ala and meso-DAP. In contrast, peptidoglycans of the genera Catenuloplanes and Couchioplanes consisted of a MurNGlyc-Gly-D-Glu-L-Lys-D-Ala peptide stem, and cross-linkage between D-Ala and L-Lys was mediated by an L-Ser residue. This method can be used to analyze the cell-wall peptidoglycan structure of other bacteria as well. By derivatization with FDLA or PMP followed by LC/MS analysis, the structure can be determined using only 0.2 mg of purified peptidoglycan.
|
| 巻・号 |
62(4)
|
| ページ |
199-205
|
| 公開日 |
2016-9-12
|
| DOI |
10.2323/jgam.2016.02.007
|
| PMID |
27477252
|
| MeSH |
Antipyrine / analogs & derivatives*
Antipyrine / chemistry
Cell Wall / chemistry*
Chemistry Techniques, Analytical
Chromatography, Liquid / methods
Edaravone
Hydrolysis
Leucine / analogs & derivatives
Leucine / chemistry
Mass Spectrometry
Micromonospora / chemistry*
Micromonospora / cytology
Micromonosporaceae / chemistry*
Micromonosporaceae / cytology
Muramic Acids / metabolism
Nitro Compounds / chemistry
Peptidoglycan / chemistry*
Peptidoglycan / isolation & purification
|
| IF |
1.442
|
| 引用数 |
9
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 3232 |
