Reference - Detail
| RRC ID | 4846 |
|---|---|
| Author | Lohmeier-Vogel EM, Kerk D, Nimick M, Wrobel S, Vickerman L, Muench DG, Moorhead GB. |
| Title | Arabidopsis At5g39790 encodes a chloroplast-localized, carbohydrate-binding, coiled-coil domain-containing putative scaffold protein. |
| Journal | BMC Plant Biol |
| Abstract |
BACKGROUND:Starch accumulation and degradation in chloroplasts is accomplished by a suite of over 30 enzymes. Recent work has emphasized the importance of multi-protein complexes amongst the metabolic enzymes, and the action of associated non-enzymatic regulatory proteins. Arabidopsis At5g39790 encodes a protein of unknown function whose sequence was previously demonstrated to contain a putative carbohydrate-binding domain. RESULTS:We here show that At5g39790 is chloroplast-localized, and binds starch, with a preference for amylose. The protein persists in starch binding under conditions of pH, redox and Mg(+2) concentrations characteristic of both the day and night chloroplast cycles. Bioinformatic analysis demonstrates a diurnal pattern of gene expression, with an accumulation of transcript during the light cycle and decline during the dark cycle. A corresponding diurnal pattern of change in protein levels in leaves is also observed. Sequence analysis shows that At5g39790 has a strongly-predicted coiled-coil domain. Similar analysis of the set of starch metabolic enzymes shows that several have strong to moderate coiled-coil potential. Gene expression analysis shows strongly correlated patterns of co-expression between At5g39790 and several starch metabolic enzymes. CONCLUSION:We propose that At5g39790 is a regulatory scaffold protein, persistently binding the starch granule, where it is positioned to interact by its coiled-coil domain with several potential starch metabolic enzyme binding-partners. |
| Volume | 8 |
| Pages | 120 |
| Published | 2008-11-27 |
| DOI | 10.1186/1471-2229-8-120 |
| PII | 1471-2229-8-120 |
| PMID | 19038037 |
| PMC | PMC2653042 |
| MeSH | Amino Acid Sequence Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis Proteins / genetics Arabidopsis Proteins / isolation & purification Arabidopsis Proteins / metabolism* Chloroplasts / metabolism* Cloning, Molecular Gene Expression Regulation, Plant Molecular Sequence Data Oligonucleotide Array Sequence Analysis Protein Binding Sequence Alignment Starch / metabolism* Substrate Specificity Time Factors |
| IF | 3.497 |
| Times Cited | 17 |
| WOS Category | PLANT SCIENCES |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pda04554 |