| RRC ID |
48493
|
| 著者 |
Maekawa T, Ishijima AS, Ida M, Izumo T, Ono Y, Shibata H, Abe S.
|
| タイトル |
Prophylactic Effect of Lactobacillus pentosus strain S-PT84 on Candida Infection and Gastric Inflammation in a Murine Gastrointestinal Candidiasis Model.
|
| ジャーナル |
Med Mycol J
|
| Abstract |
We previously showed a prophylactic effect of Lactobacillus pentosus strain S-PT84 against oral candidiasis in mice. In the present study, we evaluated the protective effect of S-PT84 against Candida infection of the gastrointestinal tract. As the first step, we used an in vitro assay to compare the inhibitory effects of several lactobacilli (S-PT84 and Lactobacillus pentosus type strain JCM1558T, Lactobacillus gasseri type strain JCM1131T and Lactobacillus casei type strain JCM1134T) on mycelial growth of Candida albicans. S-PT84 directly adhered to Candida cells and showed the strongest growth-inhibitory activity among the tested Lactobacillus strains. In the second experiment, we used an in vivo assay to evaluate the effect of S-PT84 ingestion on severity score of stomach lesion and gastric inflammation in a mouse model of gastrointestinal candidiasis. The severity scores were significantly improved by oral administration of S-PT84 (6 mg/ 200 μL), consistent with decreased coverage of stomach lesions by patchy whitish plaques. The attenuation of stomach lesion severity by S-PT84 was more pronounced than that obtained with L. gasseri type strain JCM1131T, consistent with the results of the above in vitro study. Histological analysis also indicated that S-PT84 prevented the adhesion of C. albicans to the stomach surface and suppressed stomach inflammation caused by neutrophil infiltration. Furthermore, S-PT84 also suppressed the vascular permeability observed in Candida-infected stomach. These results suggest that oral administration of S-PT84 might be effective not only in inhibiting Candida infection but also in preventing gastric inflammation induced by Candida infection.
|
| 巻・号 |
57(4)
|
| ページ |
E81-E92
|
| 公開日 |
2016-1-1
|
| DOI |
10.3314/mmj.16-00012
|
| PMID |
27904056
|
| MeSH |
Administration, Oral
Animals
Antibiosis*
Candida albicans / growth & development*
Candidiasis / prevention & control*
Capillary Permeability
Disease Models, Animal
Female
Gastrointestinal Diseases / prevention & control*
Inflammation / prevention & control*
Lactobacillus pentosus*
Mice, Inbred ICR
Neutrophil Infiltration*
Probiotics / administration & dosage*
Severity of Illness Index
Stomach / microbiology
|
| リソース情報 |
| 一般微生物 |
JCM 1558
JCM 1131
JCM 1134 |
