| RRC ID |
48499
|
| 著者 |
Mitsunobu S, Zhu M, Takeichi Y, Ohigashi T, Suga H, Jinno M, Makita H, Sakata M, Ono K, Mase K, Takahashi Y.
|
| タイトル |
Direct Detection of Fe(II) in Extracellular Polymeric Substances (EPS) at the Mineral-Microbe Interface in Bacterial Pyrite Leaching.
|
| ジャーナル |
Microbes Environ
|
| Abstract |
We herein investigated the mechanisms underlying the contact leaching process in pyrite bioleaching by Acidithiobacillus ferrooxidans using scanning transmission X-ray microscopy (STXM)-based C and Fe near edge X-ray absorption fine structure (NEXAFS) analyses. The C NEXAFS analysis directly showed that attached A. ferrooxidans produces polysaccharide-abundant extracellular polymeric substances (EPS) at the cell-pyrite interface. Furthermore, by combining the C and Fe NEXAFS results, we detected significant amounts of Fe(II), in addition to Fe(III), in the interfacial EPS at the cell-pyrite interface. A probable explanation for the Fe(II) in detected EPS is the leaching of Fe(II) from the pyrite. The detection of Fe(II) also indicates that Fe(III) resulting from pyrite oxidation may effectively function as an oxidizing agent for pyrite at the cell-pyrite interface. Thus, our results imply that a key role of Fe(III) in EPS, in addition to its previously described role in the electrostatic attachment of the cell to pyrite, is enhancing pyrite dissolution.
|
| 巻・号 |
31(1)
|
| ページ |
63-9
|
| 公開日 |
2016-1-1
|
| DOI |
10.1264/jsme2.ME15137
|
| PMID |
26947441
|
| PMC |
PMC4791118
|
| MeSH |
Acidithiobacillus / metabolism*
Biopolymers / chemistry*
Biopolymers / metabolism*
Electron Probe Microanalysis
Environmental Microbiology*
Iron / analysis*
Iron / metabolism
Microscopy, Electron, Scanning Transmission
Oxidation-Reduction
Sulfides / metabolism*
|
| IF |
2.575
|
| 引用数 |
9
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 7812 |
