論文 - 詳細
| RRC ID | 4850 |
|---|---|
| 著者 | Kawada T, Aoyama M, Okada I, Sakai T, Sekiguchi T, Ogasawara M, Satake H. |
| タイトル | A novel inhibitory gonadotropin-releasing hormone-related neuropeptide in the ascidian, Ciona intestinalis. |
| ジャーナル | Peptides |
| Abstract |
The gonadotropin-releasing hormone (GnRH) family peptides are most widely distributed neuropeptides and/or neurophysial hormones. GnRH is involved in diverse neuroendocrine, paracrine, autocrine, and neurotransmitter/neuromodulatory functions in the central and peripheral nervous system as well as peripheral tissues. In the present study, we show the identification of a novel GnRH-related peptide, Ci-GnRH-X, in the ascidian, Ciona intestinalis. Intriguingly, Ci-GnRH-X possesses a unique primary sequence consisting of 16 amino acids, although typical GnRH family peptides are composed of 10 amino acids. On the other hand, Ci-GnRH-X shares the GnRH consensus motifs, including the N-terminal pQHWS ('pQ' indicates a pyro-glutamic acid) and C-terminal Gly-amide. Reverse transcription (RT)-PCR analysis shows that the Ci-GnRH-X gene is expressed exclusively in the central nervous system. Moreover, in situ hybridization demonstrated that the Ciona GnRH-1 gene encoding Ciona GnRHs (t-GnRH-3, -5 and -6) was co-expressed with the Ci-GnRH-X gene in neurons of the cerebral ganglion. Of particular interest is that Ci-GnRH-X exhibited moderate (10-50%) inhibitory activity against t-GnRHs at their cognate receptors. Ci-GnRH-X repressed the elevation of the intracellular calcium and cAMP production by t-GnRH-6 at Ci-GnRHR-1, and cAMP production by t-GnRH-3, and t-GnRH-5 via Ci-GnRHR-3 was also inhibited by Ci-GnRH-X. In contrast, no inhibitory effect of Ci-GnRH-X at Ci-GnRHR-2 was observed. The localization and biochemical assays revealed that Ci-GnRH-X acts as an endogenous antagonist for the Ciona GnRHergic system. This is the first molecular and functional characterization of an endogenous inhibitor of GnRHs in an animal species. |
| 巻・号 | 30(12) |
| ページ | 2200-5 |
| 公開日 | 2009-12-1 |
| DOI | 10.1016/j.peptides.2009.08.014 |
| PII | S0196-9781(09)00350-7 |
| PMID | 19712719 |
| MeSH | Amino Acid Sequence Animals Calcium / metabolism Cell Line Ciona intestinalis / genetics Ciona intestinalis / metabolism* Cyclic AMP / metabolism Gene Expression Regulation Gonadotropin-Releasing Hormone / antagonists & inhibitors* Humans In Situ Hybridization Mass Spectrometry Molecular Sequence Data Neuropeptides / chemistry Neuropeptides / genetics Neuropeptides / metabolism* Neuropeptides / pharmacology* Reverse Transcriptase Polymerase Chain Reaction |
| IF | 2.843 |
| 引用数 | 18 |
| WOS 分野 | PHARMACOLOGY & PHARMACY ENDOCRINOLOGY & METABOLISM BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| カタユウレイボヤ・(ニッポンウミシダ) | Wild C. int |