RRC ID 48503
Author Harata G, He F, Takahashi K, Hosono A, Miyazawa K, Yoda K, Hiramatsu M, Kaminogawa S.
Title Human Lactobacillus Strains from the Intestine can Suppress IgE-Mediated Degranulation of Rat Basophilic Leukaemia (RBL-2H3) Cells.
Journal Microorganisms
Abstract Mast cells play a critical role in immunoglobulin E (IgE)-mediated allergic diseases, and the degranulation of mast cells is important in the pathogenesis of these diseases. A disturbance of the intestinal microflora, especially of endogenous lactic acid bacteria, might be a contributing factor for IgE-mediated allergic diseases. Additional knowledge regarding the interaction of human intestinal Lactobacilli with mast cells is still necessary. Twenty-three strains of Lactobacilli, including commercial and reference strains and strains from the human intestine, were tested for their ability to regulate degranulation of cells from rat basophilic leukemia RBL-2H3 cells (RBL-2H3) in vitro based on a β-hexosaminidase release assay. Each of the tested Lactobacilli characteristically suppressed IgE-mediated degranulation of RBL-2H3 cells, and Lactobacillus GG showed the strongest inhibitory effect on the cells. Furthermore, the bacteria isolated from the human intestine significantly suppressed degranulation of RBL-2H3 cellsin comparison with the reference strains. These results suggest that Lactobacilli, particularly those from the human intestine, can affect the activation of mast cells in a strain-dependent manner. Further study should be conducted to analyse the understanding mechanism.
Volume 4(4)
Published 2016-10-27
DOI 10.3390/microorganisms4040040
PII microorganisms4040040
PMID 27801804
PMC PMC5192523
IF 4.152
General Microbes JCM 1059 JCM 1112 JCM 1131 JCM 1132 JCM 1134 JCM 1136 JCM 1149 JCM 1231 JCM 2012