論文 - 詳細
| RRC ID | 48539 |
|---|---|
| 著者 | Tegtmeier D, Riese C, Geissinger O, Radek R, Brune A. |
| タイトル | Breznakia blatticola gen. nov. sp. nov. and Breznakia pachnodae sp. nov., two fermenting bacteria isolated from insect guts, and emended description of the family Erysipelotrichaceae. |
| ジャーナル | Syst Appl Microbiol |
| Abstract |
Two novel, obligately anaerobic Firmicutes from the family Erysipelotrichaceae were isolated from the intestinal tracts of a cockroach (strain ErySL, Shelfordella lateralis) and a scarab beetle larva (strain Pei061, Pachnoda ephippiata). Phylogenetic analysis indicated that the strains belong to a monophyletic group of hitherto uncultured bacteria from insect guts that are only distantly related to any described species (<90% 16S rRNA gene sequence similarity). Ultrastructural analysis revealed a Gram-positive cell envelope and, in the case of strain ErySL, a wide electron-lucent space between the cytoplasmic membrane and cell wall. In older cultures, cells formed pleomorphic rods with a thicker peptidoglycan layer. Both strains were obligately anaerobic and fermented glucose to formate, ethanol, and acetate as major products, but strain Pei061 tolerated up to 1% oxygen in the headspace. The same type of metabolism was observed with Erysipelothrix inopinata, except that the latter grew, albeit poorly, even under air. However, previous claims of a microaerophilic or facultatively anaerobic metabolism in the genus Erysipelothrix could not be substantiated. Based on phenotypic and phylogenetic evidence, we propose to classify the isolates as members of a new genus, Breznakia blatticola gen. nov. sp. nov. and Breznakia pachnodae sp. nov., with strain ErySL(T) (=DSM 28867(T)=JCM 30190(T)) and strain Pei061(T) (=DSM 16784(T)=JCM 30191(T)) as type strains, and provide an emended description of the family Erysipelotrichaceae. |
| 巻・号 | 39(5) |
| ページ | 319-29 |
| 公開日 | 2016-7-1 |
| DOI | 10.1016/j.syapm.2016.05.003 |
| PII | S0723-2020(16)30022-4 |
| PMID | 27270136 |
| MeSH | Animals Bacterial Typing Techniques Base Composition / genetics Base Sequence Coleoptera / microbiology* DNA, Bacterial / genetics Fatty Acids / metabolism Fermentation / physiology* Firmicutes* / classification Firmicutes* / genetics Firmicutes* / isolation & purification Gastrointestinal Microbiome / genetics* Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA |
| IF | 3.224 |
| 引用数 | 10 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 30190 JCM 30191 |