| RRC ID |
48540
|
| 著者 |
Gao F, Al-Saari N, Rohul Amin AK, Sato K, Mino S, Suda W, Oshima K, Hattori M, Ohkuma M, Hargreaves PI, Meirelles PM, Thompson FL, Thompson C, Gomez-Gil B, Sawabe T, Sawabe T.
|
| タイトル |
Vibrio ishigakensis sp. nov., in Halioticoli clade isolated from seawater in Okinawa coral reef area, Japan.
|
| ジャーナル |
Syst Appl Microbiol
|
| Abstract |
Five novel strains showing non-motile, alginolytic, halophilic and fermentative features were isolated from seawater samples off Okinawa in coral reef areas. These strains were characterized by an advanced polyphasic taxonomy including genome based taxonomy using multilocus sequence analysis (MLSA) and in silico DNA-DNA similarity (in silico DDH). Phylogenetic analyses on the basis of 16S rRNA gene sequences revealed that the isolates could be assigned to the genus Vibrio, however they were not allocated into any distinct cluster with known Vibrionaceae species. MLSA based on eight protein-coding genes (gapA, gyrB, ftsZ, mreB, pyrH, recA, rpoA, and topA) showed the vibrios formed an outskirt branch of Halioticoli clade. The experimental DNA-DNA hybridization data revealed that the five strains were in the range of being defined as conspecific but separate from nine Halioticoli clade species. The G+C contents of the Vibrio ishigakensis strains were 47.3-49.1mol%. Both Amino Acid Identity and Average Nucleotide Identity of the strain C1(T) against Vibrio ezurae HDS1-1(T), Vibrio gallicus HT2-1(T), Vibrio halioticoli IAM 14596(T), Vibrio neonatus HDD3-1(T) and Vibrio superstes G3-29(T) showed less than 95% similarity. The genome-based taxonomic approach by means of in silico DDH values also supports the V. ishigakensis strains being distinct from the other known Halioticoli clade species. Sixteen traits (growth temperature range, DNase and lipase production, indole production, and assimilation of 10 carbon compounds) distinguished these strains from Halioticoli clade species. The names V. ishigakensis sp. nov. is proposed for the species of Halioticoli clade, with C1(T) as the type strain (JCM 19231(T)=LMG 28703(T)).
|
| 巻・号 |
39(5)
|
| ページ |
330-5
|
| 公開日 |
2016-7-1
|
| DOI |
10.1016/j.syapm.2016.04.002
|
| PII |
S0723-2020(16)30003-0
|
| PMID |
27262360
|
| MeSH |
Alginates / metabolism
Animals
Anthozoa / microbiology*
Base Composition / genetics
Base Sequence
Coral Reefs*
DNA, Bacterial / genetics
Genes, Essential / genetics
Japan
Multilocus Sequence Typing
Nucleic Acid Hybridization
Phylogeny
RNA, Ribosomal, 16S / genetics
Seawater / microbiology*
Sequence Analysis, DNA
Vibrio* / classification
Vibrio* / genetics
Vibrio* / isolation & purification
|
| IF |
3.224
|
| 引用数 |
7
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 19231
JCM 19232
JCM 19241
JCM 15146 |
