RRC ID 48580
Author Hirano Y, Nakagawa M, Suyama T, Murase K, Shirakawa M, Takayama S, Sun TP, Hakoshima T.
Title Structure of the SHR-SCR heterodimer bound to the BIRD/IDD transcriptional factor JKD.
Journal Nat Plants
Abstract The plant-specific GAI, RGA and SCR (GRAS) family proteins play critical roles in plant development and signalling. Two GRAS proteins, SHORT-ROOT (SHR) and SCARECROW (SCR), cooperatively direct asymmetric cell division and the patterning of root cell types by transcriptional control in conjunction with BIRD/INDETERMINATE DOMAIN (IDD) transcription factors, although precise details of these specific interactions and actions remain unknown. Here, we present the crystal structures of the SHR-SCR binary and JACKDAW (JKD)/IDD10-SHR-SCR ternary complexes. Each GRAS domain comprises one α/β core subdomain with an α-helical cap that mediates heterodimerization by forming an intermolecular helix bundle. The α/β core subdomain of SHR forms the BIRD binding groove, which specifically recognizes the zinc fingers of JKD. We identified a conserved SHR-binding motif in 13 BIRD/IDD transcription factors. Our results establish a structural basis for GRAS-GRAS and GRAS-BIRD interactions and provide valuable clues towards our understanding of these regulators, which are involved in plant-specific signalling networks.
Volume 3
Pages 17010
Published 2017-2-17
DOI 10.1038/nplants.2017.10
PII nplants201710
PMID 28211915
PMC PMC5639936
MeSH Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Carrier Proteins / chemistry Carrier Proteins / genetics* Carrier Proteins / metabolism Transcription Factors / chemistry Transcription Factors / genetics* Transcription Factors / metabolism
IF 13.256
Times Cited 21
WOS Category PLANT SCIENCES
Resource
Arabidopsis / Cultured plant cells, genes pda08270 pda04699