| RRC ID |
4863
|
| 著者 |
Sekiguchi T, Suzuki N, Fujiwara N, Aoyama M, Kawada T, Sugase K, Murata Y, Sasayama Y, Ogasawara M, Satake H.
|
| タイトル |
Calcitonin in a protochordate, Ciona intestinalis--the prototype of the vertebrate calcitonin/calcitonin gene-related peptide superfamily.
|
| ジャーナル |
FEBS J
|
| Abstract |
The calcitonin (CT)/CT gene-related peptides (CGRPs) constitute a large peptide family in vertebrates. However, no CT/CGRP superfamily members have so far been identified in invertebrates, and the evolutionary process leading to the diverse vertebrate CT/CGRP superfamily members remains unclear. In this study, we have identified an authentic invertebrate CT, Ci-CT, in the ascidian Ciona intestinalis, which is the phylogenetically closest invertebrate chordate to vertebrates. The amino acid sequence of Ci-CT was shown to display high similarity to those of vertebrate CTs and to share CT consensus motifs, including the N-terminal circular region and C-terminal amidated proline. Furthermore, the Ci-CT gene was found to be the only Ciona CT/CGRP superfamily gene. Ci-CT also exhibited less potent, but significant, activation of the human CT receptor, as compared with salmon CT. Physiological analysis revealed that Ci-CT reduced the osteoclastic activity that is specific to vertebrate CTs. CD analysis demonstrated that Ci-CT weakly forms an alpha-helix structure. These results provide evidence that the CT/CGRP superfamily is essentially conserved in ascidians as well as in vertebrates, and indicate that Ci-CT is a prototype of vertebrate CT/CGRP superfamily members. Moreover, expression analysis demonstrated that Ci-CT is expressed in more organs than vertebrate CTs in the cognate organs, suggesting that an original CT/CGRP superfamily member gene was also expressed in multiple organs, and each CT/CGRP superfamily member acquired its current specific tissue distribution and physiological role concomitantly with diversification of the CT/CGRP superfamily during the evolution of chordates. This is the first report on a CT/CGRP superfamily member in invertebrates.
|
| 巻・号 |
276(16)
|
| ページ |
4437-47
|
| 公開日 |
2009-8-1
|
| DOI |
10.1111/j.1742-4658.2009.07151.x
|
| PMID |
19691140
|
| MeSH |
Amino Acid Sequence
Animals
Calcitonin / chemistry*
Calcitonin / genetics
Calcitonin / metabolism
Calcitonin Gene-Related Peptide
Chordata
Ciona intestinalis / chemistry*
Humans
Organ Specificity
Protein Structure, Secondary
Receptors, Calcitonin / metabolism
Salmon
Species Specificity
Vertebrates
|
| IF |
4.392
|
| 引用数 |
37
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カタユウレイボヤ・(ニッポンウミシダ) |
Wild C.int |
