RRC ID 48696
Author Xuan H, Dai X, Li J, Zhang X, Yang C, Luo F.
Title A Bacillus sp. strain with antagonistic activity against Fusarium graminearum kills Microcystis aeruginosa selectively.
Journal Sci Total Environ
Abstract Cyanobacterial harmful algal blooms (CyanoHABs) cause severe environmental problems, economic losses and threaten human health seriously. In the present study, a Bacillus sp. strain, designated as AF-1, with strong antagonistic activity against plant pathogenic fungus Fusarium graminearum was isolated from purple soil. Bacillus sp. AF-1 selectively killed Microcystis aeruginosa at low cell density (1.6×103cfu/mL), and showed the strongest bactericidal activity against M. aeruginosa NIES-843 (Ae=93%, t=6d). The algicidal substances originated from strain AF-1 were stable in the temperature range of 35-100°C, and pH range of 3-11. Cell-free filtrate of AF-1 culture caused excessive accumulation of intracellular reactive oxygen species (ROS), cell death and the efflux of intracellular components of M. aeruginosa NIES-843 cells. The expression of genes recA, psbA1, psbD1, rbcL and mcyB, involved in DNA repair, photosynthesis and microcystin synthesis of NIES 843, were significantly influenced by the cell-free filtrate of AF-1 culture. Bacillus sp. AF-1 has the potential to be developed as a bifunctional biocontrol agent to control CyanoHABs and F. graminearum caused plant disease.
Volume 583
Pages 214-221
Published 2017-4-1
DOI 10.1016/j.scitotenv.2017.01.055
PII S0048-9697(17)30055-4
PMID 28104332
MeSH Bacillus / physiology* Fusarium / physiology* Harmful Algal Bloom / physiology* Microcystis / physiology* Water Pollution / prevention & control*
IF 6.551
Times Cited 8
Algae NIES-44 NIES-90 NIES-843