論文 - 詳細
| RRC ID | 48703 |
|---|---|
| 著者 | Reichert M, Bergmann SM, Hwang J, Buchholz R, Lindenberger C. |
| タイトル | Antiviral activity of exopolysaccharides from Arthrospira platensis against koi herpesvirus. |
| ジャーナル | J Fish Dis |
| Abstract |
Although koi herpesvirus (KHV) has a history of causing severe economic losses in common carp and koi farms, there are still no treatments available on the market. Thus, the aim of this study was to test exopolysaccharides (EPS) for its antiviral activity against KHV, by monitoring inhibition and cytotoxic effects in common carp brain cells. These substances can be easily extracted from extracellular algae supernatant and were identified as groups of sulphated polysaccharides. In order to reach this aim, Arthrospira platensis, which is well known for its antiviral activity of intra- and extracellular compounds towards mammalian herpesviruses, was investigated as standard organism and compared to commercial antiviral drug, ganciclovir, which inhibits the viral DNA polymerization. The antiviral activity of polysaccharides of A. platensis against KHV was confirmed in vitro using qualitative assessment of KHV life cycle genes, and it was found by RT-PCR that EPS, applied at a concentration of >18 μg mL-1 and a multiplicity of infection (MOI) of 0.45 of KHV, suppressed the viral replication in common carp brain (CCB) cells even after 22 days post-infection, entirely. Further, this study presents first data indicating an enormous potential using polysaccharides as an additive for aquacultures to lower or hinder the spread of the KHV and koi herpesvirus disease (KHVD) in future. |
| 巻・号 | 40(10) |
| ページ | 1441-1450 |
| 公開日 | 2017-10-1 |
| DOI | 10.1111/jfd.12618 |
| PMID | 28422294 |
| MeSH | Animals Antiviral Agents / pharmacology* Brain / virology Carps* Dose-Response Relationship, Drug Fish Diseases / virology* Herpesviridae / drug effects* Herpesviridae Infections / veterinary* Herpesviridae Infections / virology Polysaccharides, Bacterial / chemistry Polysaccharides, Bacterial / pharmacology* Spirulina / chemistry* |
| IF | 2.318 |
| 引用数 | 7 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-39 |