RRC ID 48719
Author Weimer AK, Biedermann S, Harashima H, Roodbarkelari F, Takahashi N, Foreman J, Guan Y, Pochon G, Heese M, Van Damme D, Sugimoto K, Koncz C, Doerner P, Umeda M, Schnittger A.
Title The plant-specific CDKB1-CYCB1 complex mediates homologous recombination repair in Arabidopsis.
Journal EMBO J
Abstract Upon DNA damage, cyclin-dependent kinases (CDKs) are typically inhibited to block cell division. In many organisms, however, it has been found that CDK activity is required for DNA repair, especially for homology-dependent repair (HR), resulting in the conundrum how mitotic arrest and repair can be reconciled. Here, we show that Arabidopsis thaliana solves this dilemma by a division of labor strategy. We identify the plant-specific B1-type CDKs (CDKB1s) and the class of B1-type cyclins (CYCB1s) as major regulators of HR in plants. We find that RADIATION SENSITIVE 51 (RAD51), a core mediator of HR, is a substrate of CDKB1-CYCB1 complexes. Conversely, mutants in CDKB1 and CYCB1 fail to recruit RAD51 to damaged DNA CYCB1;1 is specifically activated after DNA damage and we show that this activation is directly controlled by SUPPRESSOR OF GAMMA RESPONSE 1 (SOG1), a transcription factor that acts similarly to p53 in animals. Thus, while the major mitotic cell-cycle activity is blocked after DNA damage, CDKB1-CYCB1 complexes are specifically activated to mediate HR.
Volume 35(19)
Pages 2068-2086
Published 2016-10-4
DOI 10.15252/embj.201593083
PII embj.201593083
PMID 27497297
PMC PMC5048351
MeSH Arabidopsis / genetics* Arabidopsis / metabolism* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cyclin B / genetics Cyclin B / metabolism* Cyclin-Dependent Kinases / genetics Cyclin-Dependent Kinases / metabolism* Rad51 Recombinase / metabolism Recombinational DNA Repair* Transcription Factors / metabolism
IF 9.889
Times Cited 40
Arabidopsis / Cultured plant cells, genes pst15850