RRC ID 48770
Author Tschopp MA, Iki T, Brosnan CA, Jullien PE, Pumplin N.
Title A complex of Arabidopsis DRB proteins can impair dsRNA processing.
Journal RNA
Abstract mutant plants, suggesting that DRB7.1 can antagonize the function of DRB4 in siRNA accumulation in vivo as well as in vitro. Specifically, enhanced accumulation of siRNAs from an endogenous inverted repeat correlated with enhanced DNA methylation, suggesting a biological impact for DRB7.1 in regulating epigenetic marks. We further demonstrate that RNase three-like (RTL) proteins RTL1 and RTL2 cleave dsRNA when expressed in BYL, and that this activity is impaired by DRB7.1/DRB4. Investigating the DRB7.1-DRB4 interaction thus revealed that a complex of DRB proteins can antagonize, rather than promote, RNase III activity and production of siRNAs in plants.
Volume 23(5)
Pages 782-797
Published 2017-5
DOI 10.1261/rna.059519.116
PII rna.059519.116
PMID 28232389
PMC PMC5393186
MeSH Arabidopsis Proteins / metabolism* Gene Expression Regulation, Plant RNA Processing, Post-Transcriptional* RNA, Double-Stranded / metabolism* RNA, Small Interfering / metabolism* RNA-Binding Proteins / metabolism* Repressor Proteins / metabolism Ribonuclease III / metabolism
IF 4.49
Times Cited 0
Arabidopsis / Cultured plant cells, genes pst18218