Reference - Detail
| RRC ID | 48774 |
|---|---|
| Author | Kurita K, Sakamoto T, Yagi N, Sakamoto Y, Ito A, Nishino N, Sako K, Yoshida M, Kimura H, Seki M, Matsunaga S. |
| Title | Live imaging of H3K9 acetylation in plant cells. |
| Journal | Sci Rep |
| Abstract |
Proper regulation of histone acetylation is important in development and cellular responses to environmental stimuli. However, the dynamics of histone acetylation at the single-cell level remains poorly understood. Here we established a transgenic plant cell line to track histone H3 lysine 9 acetylation (H3K9ac) with a modification-specific intracellular antibody (mintbody). The H3K9ac-specific mintbody fused to the enhanced green fluorescent protein (H3K9ac-mintbody-GFP) was introduced into tobacco BY-2 cells. We successfully demonstrated that H3K9ac-mintbody-GFP interacted with H3K9ac in vivo. The ratio of nuclear/cytoplasmic H3K9ac-mintbody-GFP detected in quantitative analysis reflected the endogenous H3K9ac levels. Under chemically induced hyperacetylation conditions with histone deacetylase inhibitors including trichostatin A, Ky-2 and Ky-14, significant enhancement of H3K9ac was detected by H3K9ac-mintbody-GFP dependent on the strength of inhibitors. Conversely, treatment with a histone acetyltransferase inhibitor, C646 caused a reduction in the nuclear to cytoplasmic ratio of H3K9ac-mintbody-GFP. Using this system, we assessed the environmental responses of H3K9ac and found that cold and salt stresses enhanced H3K9ac in tobacco BY-2 cells. In addition, a combination of H3K9ac-mintbody-GFP with 5-ethynyl-2'-deoxyuridine labelling confirmed that H3K9ac level is constant during interphase. |
| Volume | 7 |
| Pages | 45894 |
| Published | 2017-4-18 |
| DOI | 10.1038/srep45894 |
| PII | srep45894 |
| PMID | 28418019 |
| PMC | PMC5394682 |
| MeSH | Acetylation / drug effects Antibodies / immunology Antibodies / metabolism Benzoates / pharmacology Green Fluorescent Proteins / chemistry Histone Acetyltransferases / genetics* Histone Acetyltransferases / immunology Histone Acetyltransferases / ultrastructure Histone Deacetylase Inhibitors / pharmacology Histones / immunology Nitrobenzenes Plant Cells / metabolism Plant Cells / ultrastructure* Plants, Genetically Modified Protein Processing, Post-Translational / genetics* Pyrazoles / pharmacology Pyrazolones Tobacco / genetics Tobacco / growth & development Tobacco / metabolism* |
| IF | 3.998 |
| Times Cited | 3 |
| WOS Category | MULTIDISCIPLINARY SCIENCES |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | rpc00001 |