RRC ID 48781
Author Kurita R, Suda N, Sudo K, Miharada K, Hiroyama T, Miyoshi H, Tani K, Nakamura Y.
Title Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells.
Journal PLoS One
Abstract Transfusion of red blood cells (RBCs) is a standard and indispensable therapy in current clinical practice. In vitro production of RBCs offers a potential means to overcome a shortage of transfusable RBCs in some clinical situations and also to provide a source of cells free from possible infection or contamination by microorganisms. Thus, in vitro production of RBCs may become a standard procedure in the future. We previously reported the successful establishment of immortalized mouse erythroid progenitor cell lines that were able to produce mature RBCs very efficiently. Here, we have developed a reliable protocol for establishing immortalized human erythroid progenitor cell lines that are able to produce enucleated RBCs. These immortalized cell lines produce functional hemoglobin and express erythroid-specific markers, and these markers are upregulated following induction of differentiation in vitro. Most importantly, these immortalized cell lines all produce enucleated RBCs after induction of differentiation in vitro, although the efficiency of producing enucleated RBCs remains to be improved further. To the best of our knowledge, this is the first demonstration of the feasibility of using immortalized human erythroid progenitor cell lines as an ex vivo source for production of enucleated RBCs.
Volume 8(3)
Pages e59890
Published 2013-1-1
DOI 10.1371/journal.pone.0059890
PII PONE-D-12-35323
PMID 23533656
PMC PMC3606290
MeSH Cell Nucleus* Erythrocyte Transfusion / methods Erythrocytes / cytology* Erythroid Precursor Cells / cytology* Erythropoiesis / physiology* Humans
IF 2.74
Times Cited 130
DNA material CSIV-TRE-RfA-CMV-KT (RDB12876) CSIV-TRE-RfA-UbC-KT (RDB12878) CSII-EF-RfA-IRES-Puro (RDB12869) CSIV-TRE-HPV-E6/E7-UbC-KT (RDB17719)
Human and Animal Cells HiPS-RIKEN-3A(HPS0015) HiPS-RIKEN-4A(HPS0021) OP9(RCB1124)
Cord blood stem cells for research