論文 - 詳細
| RRC ID | 48782 |
|---|---|
| 著者 | Miura T, Moriya H, Iwai S. |
| タイトル | Assessing phagotrophy in the mixotrophic ciliate Paramecium bursaria using GFP-expressing yeast cells. |
| ジャーナル | FEMS Microbiol Lett |
| Abstract |
We used cells of the yeast Saccharomyces cerevisiae expressing green fluorescent protein (GFP) as fluorescently labelled prey to assess the phagocytic activities of the mixotrophic ciliate Paramecium bursaria, which harbours symbiotic Chlorella-like algae. Because of different fluorescence spectra of GFP and algal chlorophyll, ingested GFP-expressing yeast cells can be distinguished from endosymbiotic algal cells and directly counted in individual P. bursaria cells using fluorescence microscopy. By using GFP-expressing yeast cells, we found that P. bursaria altered ingestion activities under different physiological conditions, such as different growth phases or the presence/absence of endosymbionts. Use of GFP-expressing yeast cells allowed us to estimate the digestion rates of live prey of the ciliate. In contrast to the ingestion activities, the digestion rate within food vacuoles was not affected by the presence of endosymbionts, consistent with previous findings that food and perialgal vacuoles are spatially and functionally separated in P. bursaria. Thus, GFP-expressing yeast may provide a valuable tool to assess both ingestion and digestion activities of ciliates that feed on eukaryotic organisms. |
| 巻・号 | 364(12) |
| 公開日 | 2017-6-7 |
| DOI | 10.1093/femsle/fnx117 |
| PII | 3861962 |
| PMID | 28591835 |
| MeSH | Chlorella vulgaris / physiology Green Fluorescent Proteins / genetics* Paramecium / genetics* Paramecium / growth & development Paramecium / physiology* Phagocytosis* Photosynthesis Saccharomyces cerevisiae / genetics* Symbiosis Vacuoles |
| IF | 1.994 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゾウリムシ | HA1g (PB034004A) |