RRC ID 48783
Author Arae T, Isai S, Sakai A, Mineta K, Yokota Hirai M, Suzuki Y, Kanaya S, Yamaguchi J, Naito S, Chiba Y.
Title Co-ordinated Regulations of mRNA Synthesis and Decay during Cold Acclimation in Arabidopsis Cells.
Journal Plant Cell Physiol.
Abstract Plants possess a cold acclimation system to acquire freezing tolerance through pre-exposure to non-freezing low temperatures. The transcriptional cascade of C-repeat-binding factors (CBFs)/dehydration response element-binding factors (DREBs) is considered a major transcriptional regulatory pathway during cold acclimation. However, little is known regarding the functional significance of mRNA stability regulation in the response of gene expression to cold stress. The actual level of individual mRNAs is determined by a balance between mRNA synthesis and degradation. Therefore, it is important to assess the regulatory steps to increase our understanding of gene regulation. Here, we analyzed temporal changes in mRNA amounts and half-lives in response to cold stress in Arabidopsis cell cultures based on genome-wide analysis. In this mRNA decay array method, mRNA half-life measurements and microarray analyses were combined. In addition, temporal changes in the integrated value of transcription rates were estimated from the above two parameters using a mathematical approach. Our results showed that several cold-responsive genes, including Cold-regulated 15a, were relatively destabilized, whereas the mRNA amounts were increased during cold treatment by accelerating the transcription rate to overcome the destabilization. Considering the kinetics of mRNA synthesis and degradation, this apparently contradictory result supports that mRNA destabilization is advantageous for the swift increase in CBF-responsive genes in response to cold stress.
Volume 58(6)
Pages 1090-1102
Published 2017-6-1
DOI 10.1093/pcp/pcx059
PII 3746596
PMID 28444357
MeSH Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism Cold Temperature Gene Expression Regulation, Plant / genetics Gene Expression Regulation, Plant / physiology RNA Stability / genetics RNA Stability / physiology RNA, Messenger / genetics RNA, Messenger / metabolism* Transcription Factors / genetics Transcription Factors / metabolism Transcription, Genetic / genetics Transcription, Genetic / physiology
IF 4.059
Times Cited 0
WOS Category CELL BIOLOGY PLANT SCIENCES
Resource
Arabidopsis / Cultured plant cells, genes rpc00008