Reference - Detail
| RRC ID | 48801 |
|---|---|
| Author | Wang M, Deng WW, Zhang ZZ, Yu O. |
| Title | Engineering an ABC Transporter for Enhancing Resistance to Caffeine in Saccharomyces cerevisiae. |
| Journal | J Agric Food Chem |
| Abstract |
In addressing caffeine toxicity to the producing cells, engineering a transporter that can move caffeine from cytoplasm across the cell membrane to the extracellular space, thus enhancing caffeine resistance and potentially increasing the yield in yeast, is important. An ABC-transporter bfr1 from Schizosaccharomyces pombe was cloned and transformed into S. cerevisiae, resulting in enhancing caffeine resistance. Afterward, a library of randomly mutagenized bfr1 mutants through error-prone PCR was generated. One mutant was identified with drastically increased caffeine resistance (15 mg/mL). Sequencing and structural analysis illustrated that many of the mutations occurred at the cytosolic domain. Site-directed mutagenesis of these mutations confirmed at least one amino acid that conferred enhancing caffeine resistance in the mutated bfr1. These data demonstrated engineering ABC-transporters can be an efficient way to reduce product toxicity in heterologous systems. |
| Volume | 64(42) |
| Pages | 7973-7978 |
| Published | 2016-10-26 |
| DOI | 10.1021/acs.jafc.6b03980 |
| PMID | 27696877 |
| IF | 4.192 |
| Times Cited | 1 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | News |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | S. pombe ORFeome clone SpYFH49H06 (SPW099774) |